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B-Cell Linker 抗体 (C-Term)

This anti-B-Cell Linker antibody is a 山羊 多克隆 antibody detecting B-Cell Linker in WB, ELISA, FACS 和 IF. Suitable for 人.
产品编号 ABIN184602
发货至: 中国

Quick Overview for B-Cell Linker 抗体 (C-Term) (ABIN184602)

抗原

See all B-Cell Linker (BLNK) 抗体
B-Cell Linker (BLNK)

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This B-Cell Linker antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

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    C-Term

    原理

    BLNK / SLP-65

    序列

    KDSTRLKYAV KVS

    特异性

    This antibody is expected to recognize all reported isoforms.

    交叉反应

    Cow, 犬, 人, 小鼠, Pig, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-KDSTRLKYAVKVS, from the C Terminus of the protein sequence according to NP_037446.1, NP_001107566.1.

    亚型

    IgG
  • 应用备注

    Western Blot: Approx 70-75 kDa band observed in lysates of cell line Daudi (calculated MW of 50.5 kDa according to NP_037446.1). This molecular weight is routinely observed by other sources. Recommended concentration 0.3-1 μg/mL. Primary incubation 1 hour a

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the membranes of HepG2 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of Daudi cells. Recommended concentration: 10ug/ml

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    B-Cell Linker (BLNK)

    别名

    BLNK

    背景

    BLNK, SLP-65, B-cell linker, BASH, BLNK-s, Ly57, MGC111051, SLP65, B cell linker protein, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, OTTHUMP00000020167, Src homology 2 domain-containing leuko

    基因ID

    29760, 17060

    NCBI登录号

    NP_037446, NP_001107566, NP_001245369, NP_001245370, NP_001245371

    途径

    BCR Signaling
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