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ALDH1A1 抗体 (C-Term)

Cited in 2+ publications. This 山羊 多克隆 anti-ALDH1A1 antibody specifically detects ALDH1A1 in WB, ELISA 和 IF. The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN184581
发货至: 中国
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Quick Overview for ALDH1A1 抗体 (C-Term) (ABIN184581)

抗原

See all ALDH1A1 抗体
ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

适用

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人, 小鼠, 大鼠

宿主

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山羊

克隆类型

  • 80
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多克隆

标记

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This ALDH1A1 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

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    C-Term

    原理

    ALDH1A1 (C Terminus)

    序列

    EVKTVTVKIS QKNS

    特异性

    This antibody may cross-react with ALDH1A2 (GeneID 8854) with one residue difference from this design and with ALDH2 (GeneID 217) with two residues difference from this design.

    交叉反应

    人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    C-EVKTVTVKISQKNS

    亚型

    IgG
  • 应用备注

    Western Blot: Approx 55 kDa band observed in Human, Mouse and Rat Liver lysates (calculated MW of 54.9 kDa according to NP_000680.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

    Peptide ELISA: antibody detection limit dilution 1:1000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 10μg/ml. This antibody has been successfully used in IF on Human, PMID: 32157826.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Etienne, Joanne, Catelain, Riveron, Bayer, Lafable, Punzon, Blot, Agbulut, Vilquin: "Aldehyde dehydrogenases contribute to skeletal muscle homeostasis in healthy, aging, and Duchenne muscular dystrophy patients." in: Journal of cachexia, sarcopenia and muscle, Vol. 11, Issue 4, pp. 1047-1069, (2020) (PubMed).

    Amann, Hofmann, Freudenberger, Holland-Cunz, Eichmüller, Bazhin: "Expression and activity of alcohol and aldehyde dehydrogenases in melanoma cells and in melanocytes." in: Journal of cellular biochemistry, Vol. 113, Issue 3, pp. 792-9, (2012) (PubMed).

  • 抗原

    ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

    别名

    ALDH1A1

    背景

    ALDH1A1, aldehyde dehydrogenase 1 family, member A1, ALDC, ALDH-E1, ALDH1, ALDH11, MGC2318, PUMB1, RALDH1, ALDH class 1, acetaldehyde dehydrogenase 1, aldehyde dehydrogenase 1, soluble, aldehyde dehydrogenase 1A1, aldehyde dehydrogenase, liver cytosolic

    基因ID

    216, 11668, 24188

    NCBI登录号

    NP_000680

    途径

    Dopaminergic Neurogenesis
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