BCL7A 抗体 (C-Term)
Quick Overview for BCL7A 抗体 (C-Term) (ABIN184562)
抗原
See all BCL7A 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- BCL7A
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序列
- MKLEASQQNS EEM
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特异性
- This antibody is expected to recognize both reported isoforms (NP_066273.1, NP_001019979.1).
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交叉反应
- Cow, 犬, 人, 小鼠, Pig, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-MKLEASQQNSEEM, from the C Terminus of the protein sequence according to NP_066273.1, NP_001019979.1.
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亚型
- IgG
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应用备注
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Western Blot: Approx 26 kDa band observed in Human Lymph Node lysates (calculated MW of 25.0 kDa according to NP_066273.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:4000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of Daudi cells. Recommended concentration: 10ug/ml.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- BCL7A (B-Cell CLL/lymphoma 7A (BCL7A))
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别名
- BCL7A
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背景
- BCL7A, BCL7, B-cell CLL/lymphoma 7A, B-cell CLL/lymphoma-7
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基因ID
- 605, 77045
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NCBI登录号
- NP_066273, NP_001019979
抗原
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