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BCL7A 抗体 (C-Term)

The 山羊 多克隆 anti-BCL7A antibody is suitable to detect BCL7A in samples from 人. It has been validated for WB, ELISA, IF 和 FACS.
产品编号 ABIN184562
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for BCL7A 抗体 (C-Term) (ABIN184562)

抗原

See all BCL7A 抗体
BCL7A (B-Cell CLL/lymphoma 7A (BCL7A))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This BCL7A antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

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    C-Term

    原理

    BCL7A

    序列

    MKLEASQQNS EEM

    特异性

    This antibody is expected to recognize both reported isoforms (NP_066273.1, NP_001019979.1).

    交叉反应

    Cow, 犬, 人, 小鼠, Pig, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-MKLEASQQNSEEM, from the C Terminus of the protein sequence according to NP_066273.1, NP_001019979.1.

    亚型

    IgG
  • 应用备注

    Western Blot: Approx 26 kDa band observed in Human Lymph Node lysates (calculated MW of 25.0 kDa according to NP_066273.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:4000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of Daudi cells. Recommended concentration: 10ug/ml.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    BCL7A (B-Cell CLL/lymphoma 7A (BCL7A))

    别名

    BCL7A

    背景

    BCL7A, BCL7, B-cell CLL/lymphoma 7A, B-cell CLL/lymphoma-7

    基因ID

    605, 77045

    NCBI登录号

    NP_066273, NP_001019979
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