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- 抗原 See all CBP Tag products
- CBP Tag
- 适用
- 请咨询
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CBP Tag antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 序列
- KRRWKKNFIA VSAA
- 特异性
- CBP Tag Antibody, mAb, Mouse recognizes CBP-tagged proteins.
- 产品特性
- CBP Tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes harvested from mouse immunized with CBP tag peptide conjugated to KLH.
- 纯化方法
- Protein A affinity column
- 免疫原
- CBP tag peptide conjugated to KLH
- 克隆位点
- 1D11E5
- 亚型
- IgG2b kappa
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anti-CBP Tag antibody
WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CBP Tag antibodyWB 宿主: 小鼠 Monoclonal Mix unconjugated
anti-CBP Tag antibodyWB, IP 宿主: 小鼠 Monoclonal unconjugated
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- 应用备注
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 1 µg/mL.
Immunoprecipitation: 1-5 µg/mL.
ELISA: 0. 001-0. 002 µg/mL. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.
- 浓度
- 0.5 mg/mL
- 缓冲液
- lyophilized with PBS, pH 7.4, containing 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below.
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: "A unified mechanism for intron and exon definition and back-splicing." in: Nature, Vol. 573, Issue 7774, pp. 375-380, (2020) (PubMed).
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: "A unified mechanism for intron and exon definition and back-splicing." in: Nature, Vol. 573, Issue 7774, pp. 375-380, (2020) (PubMed).
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- 抗原
- CBP Tag
- Abstract
- CBP Tag 产品
- 物质类
- Tag
- 背景
- CBP affinity tag is a useful tag in protein study which can be added to the N or C terminus of proteins of interest through DNA recombinant technology. The tag is derived from muscle myosin light-chain kinase. The tag comprises 26 amino acid residues with the molecular weight of 4 kDa and sequence of KRRWKKNFIAVSAANRFKKISSSGAL. CBP tag has the relatively high affinity for calmodulin (CaM). CBP-tagged proteins can be purified from crude cell extracts through CaM affinity resin. CBP Tag antibody is a useful tool in analysis of CBP-tagged proteins
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