Protein A 抗体
(1 reference)Quick Overview for Protein A 抗体 (ABIN7477945)
抗原
See all Protein A 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Protein A Antibody, mAb, Mouse detects natural Protein A, variety of recombinant Protein A variants and MabSelect SuReTM ligand.
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产品特性
- Protein A Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with purified recombinant Protein A.
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纯化方法
- Protein A affinity column
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免疫原
- purified recombinant Protein A
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亚型
- IgG1 kappa
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anti-Protein A antibody
适用: Staphylococcus aureus ELISA, WB, IF (p), IF (cc), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein A antibody (Rhodamine)适用: Staphylococcus aureus IF 宿主: 山羊 Polyclonal Rhodamine
anti-Protein A antibody适用: Staphylococcus aureus ELISA, WB, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Protein A antibody适用: Staphylococcus aureus ELISA, WB, IP 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Working concentrations for specific applications should be empirically determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the detection methods, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA Capture: 1-10 µg/mL
ELISA Detection: 0. 05 - 0. 2 µg/mL
Recommended antibody pairing for sandwich immunoassay: Capture with A01778 and detected by A01779,
Western Blot: 0. 5 -1 µg/mL
Other applications: user-optimized -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute the lyophilized antibody with 200 µl deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.
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浓度
- 0.5 mg/mL
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缓冲液
- lyophilized with PBS, pH 7.4, containing 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Protein A Antibody, mAb, Mouse should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below.
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: "Defective immuno- and thymoproteasome assembly causes severe immunodeficiency. ..." in: Scientific reports, Vol. 8, Issue 1, pp. 5975, (2018) (PubMed).
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: "Defective immuno- and thymoproteasome assembly causes severe immunodeficiency. ..." in: Scientific reports, Vol. 8, Issue 1, pp. 5975, (2018) (PubMed).
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- Protein A
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背景
- Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly through Fc regions. The Protein A is most commonly bound to a chromatography resin, used for production of antibody drugs or reagents. Protein A antibody can be used for quantification of Protein A in drugs and foods or for evaluation of Protein A resin. This Protein A mAb is advantageous due to its IgG1 isotype, for mouse IgG1 has weak binding capacity to Protein A
抗原
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