(1 reference)-
- 抗原 See all GST 抗体
- GST (Glutathione S Transferase (GST))
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适用
- Schistosoma japonicum
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This GST antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 特异性
- This antibody can be used to detect GST-fusion proteins in almost expression systems including bacterial expression system, mammalian expression system and other systems.
- 产品特性
- Rabbit Anti-GST-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography. The immunogen used is a recombinant version of glutathione-S-transferase from Schistosoma japonicum. Glutathione-S-transferase is an epitope tag in generating fusion proteins. Antibodies specific to fusion proteins are useful in monitoring protein expression in Western Blots as well as immunoaffinity purification of proteins expressed in E. coli. The glutathione-S-transferases are a family of enzymes that catalyze the addition of the tripeptide glutathione to endogenous and xenobiotic substrates which have electrophilic functional groups. They play an important role in the detoxication and metabolism of many xenobiotic and endobiotic compounds. The glutathione adducts produced have increased solubility in water and are subsequently enzymatically degraded to mercapturates and excreted.
- 纯化方法
- Immunoaffinity chromatography
- 免疫原
- Recombinant Glutathione-S-Transferase from Schistosoma japonicum
- 亚型
- IgG
- Top Product
- Discover our top product GST Primary Antibody
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anti-Glutathione S Transferase (GST) antibody
GST 适用: Schistosoma japonicum WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: Schistosoma japonicum WB, ELISA, IP, DB 宿主: 山羊 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: 标记 WB 宿主: 山羊 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: Schistosoma japonicum WB, IP 宿主: 小鼠 Monoclonal S-tag-05 unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: Schistosoma japonicum WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: Schistosoma japonicum IA 宿主: 小鼠 Monoclonal 3D4 unconjugated
anti-Glutathione S Transferase (GST) antibody (FITC)GST 适用: Schistosoma japonicum FACS 宿主: 兔 Polyclonal FITC
anti-Glutathione S Transferase (GST) antibody (Biotin)GST 适用: Schistosoma japonicum WB, ELISA, IHC, IM 宿主: 山羊 Polyclonal Biotin
anti-Glutathione S Transferase (GST) antibodyGST 适用: Schistosoma japonicum WB, ELISA, IP 宿主: 小鼠 Monoclonal 2F10B9 unconjugated
anti-Glutathione S Transferase (GST) (AA 1-218) antibodyGST 适用: Various Species WB, IP, DB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0. 05-0. 5 µg/mL
Western blot: 0. 1-1 µg/mL
Western Blot Using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit L00204, C is recommended. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized - 限制
- 仅限研究用
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- 溶解方式
- Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.
- 浓度
- 0.5 mg/mL
- 缓冲液
- PBS, pH 7.4 with 0.02 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- The antibody is stable for 2-3 weeks if stored at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
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: "Comprehensive analysis of yeast surface displayed cDNA library selection outputs by exon microarray to identify novel protein-ligand interactions." in: Molecular & cellular proteomics : MCP, Vol. 10, Issue 3, pp. M110.005116, (2011) (PubMed).
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: "Comprehensive analysis of yeast surface displayed cDNA library selection outputs by exon microarray to identify novel protein-ligand interactions." in: Molecular & cellular proteomics : MCP, Vol. 10, Issue 3, pp. M110.005116, (2011) (PubMed).
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- 抗原
- GST (Glutathione S Transferase (GST))
- 别名
- Glutathione-S-Transferase (GST) (GST 产品)
- 别名
- GST antibody, GST 13-13 antibody, GST13 antibody, GST13-13 antibody, GSTK1-1 antibody, hGSTK1 antibody, 1500002K10Rik antibody, Gst antibody, Ptgds2 antibody, glutathione S-transferase kappa 1 antibody, microsomal glutathione S-transferase 1 antibody, hematopoietic prostaglandin D synthase antibody, GSTK1 antibody, Mgst1 antibody, Hpgds antibody
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