DYKDDDDK Tag 抗体 (iFluor 647)
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北京 101111
Quick Overview for DYKDDDDK Tag 抗体 (iFluor 647) (ABIN1842289)
抗原
适用
宿主
克隆类型
标记
应用范围
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序列
- DYKDDDDK
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特异性
- THETM DYKDDDDK Tag Antibody [iFluor 647], mAb, Mouse recognizes N-terminal, internal and C-terminal DYKDDDDK-tagged proteins.
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纯化方法
- Protein A affinity column
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免疫原
- A synthetic peptide DYKDDDDK coupled to KLH
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亚型
- IgG2b kappa
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anti-DYKDDDDK Tag antibody
WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal FG4R unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IP 宿主: 小鼠 Monoclonal 5A8E5 unconjugated
anti-DYKDDDDK Tag antibody适用: 标记 WB, ELISA, IP, FACS, IHC, ICC 宿主: 小鼠 Monoclonal 2F11G1 unconjugated
anti-DYKDDDDK Tag antibodyWB, IP, IF, IC 宿主: 小鼠 Monoclonal unconjugated
anti-DYKDDDDK Tag antibody适用: 人 WB 宿主: 兔 Polyclonal RB0493 unconjugated
anti-DYKDDDDK Tag antibody (HRP)WB, ELISA, IF 宿主: 小鼠 Monoclonal 5A8E5 HRP
anti-DYKDDDDK Tag antibodyWB, ELISA, FACS, IHC 宿主: 小鼠 Monoclonal 29E4-G7 unconjugated
anti-DYKDDDDK Tag antibody (HRP)WB, ELISA, IP 宿主: 兔 Polyclonal HRP
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应用备注
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescent staining: 1-4 µg/mL
Flow cytometry: 1-4 µg/mL
Other applications: user-optimized -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.
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浓度
- 0.5 mg/mL
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缓冲液
- lyophilized with PBS, pH 7.4, containing 10 mg/mL BSA and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- The lyophilized product remains stable up to 2 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below.
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- DYKDDDDK Tag
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物质类
- Tag
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背景
- The DYKDDDK tag is a polypeptide of with the amino acid sequence DYKDDDDK amino acid sequence that can be added to a target protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of the protein to facilitate detection and purification. An anti-DYKDDDK tag antibody is a useful tool for the analysis of Flag-tagged proteins. THETM DYKDDDDK Tag Antibody [iFluor 647], mAb, Mouse is THETM DYKDDDDK Tag Antibody (A00187) conjugated with iFluor 647 under optimal conditions with an F/P ratio of 3-6. It is suitable for detecting the expression level of DYKDDDDK-tagged proteins. iFluor 647 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 647.
抗原
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