Immunoprecipitation: CC101 immunoprecipitates a 215 kD molecule from bovine cells, and also recognises the swine homologue of WC1, which is a 180 kD molecule. Immunohistochemistry on frozen sections. Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1 mg/mL
缓冲液
Containing 0.09 % Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
WC1 is expressed by a population of gamma/delta TCR expression cells which lack CD2, CD4 and CD8 but express CD3. WC1 expression appears to be heterogeneous and antibodies of this cluster show differing reaction patterns. In pigs the 180kD molecule is expressed by a gamma/delta TCR positive T-cell population that is also CD2-, CD4-, CD8-.Synonyms: WC1 antigen