ICAM1 抗体
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Quick Overview for ICAM1 抗体 (ABIN182014)
抗原
See all ICAM1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody recognizes the CD54 cell surface antigen, also known as intercellular adhesion molecule-1 (ICAM-1). Recent studies suggest that cross-linking of ICAM-1 using clone 1A29 induces calcium signalling. Functionally 1A29 inhibits homotypic aggregation of PHA blasts.
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交叉反应 (详细)
- Species reactivity (tested):Rat.
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纯化方法
- Affinity chromatography on Protein G
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内毒素水平
- Low Endotoxin
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免疫原
- Rat Ax cells (a HEV derived cell line). Remarks: Spleen cells from immunised BALB/c mice were fused with cells from the PAI mouse myeloma cell line.
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亚型
- IgG1
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应用备注
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Functional Assays. Western Blot. Immunoprecipitation. Immunohistochemistry on frozen sections. Flow Cytometry: 1/25 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.4 without preservatives
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))
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别名
- CD54 / ICAM1
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背景
- CD54 is a cell surface ligand of the lymphocyte integrin, LFA-1 and is known to play an important role in various cell-cell interactions in the immune system.Synonyms: ICAM-1, Intercellular adhesion molecule 1, Major Group Rhinovirus Receptor
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基因ID
- 25464
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NCBI登录号
- NP_037099
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UniProt
- Q00238
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling
抗原
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