Radioimmunological direct binding studies were performed with 125I-labelled hPL, hGH, hPRL and increasing amounts of INN-hPL-37. In addition, competitive RIA with unlabelled hGH, hPRL and hPL were used for characterization. In competitive RIA cross reactivity with hGH and hPRL was below 1 % . Direct binding demonstrated 39 % reactivity with hGH but no cross reactivity with hPRL.
纯化方法
Affinity chromatography on Protein A
免疫原
Human placental lactogen (hPL). Spleen cells from immunised mice were fused with cells of the X63-Ag8.653 myeloma cell line.