This antibody recognises the alpha chain of the CD8 homologue, a 33-35 kDa cell surface protein. Clone 11-39 recognises all polymorphic forms of chicken CD8 alpha.
纯化方法
Affinity chromatography on Protein G
免疫原
Chicken T-cells. Lymph node cells from immunised Balb/c mice were fused with cells of the SP2/0 myeloma cell line.
CD8B
适用: Cow, 山羊, 绵羊, Water Buffalo
FACS
宿主: 小鼠
Monoclonal
CC58
PE
应用备注
Flow Cytometry: Neat - 1/5, use 10 μL of the suggested working dilution to label 10^6 cells in100l. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore with 1.0 mL distilled water
浓度
0.1 mg/mL
缓冲液
PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
储存液
Sodium azide
注意事项
Use of Sodium Azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the lyophilized antibody at 2-8 °C. Store the antibody after reconstitution for several weeks at 2-8 °C. Prepare working dilution only prior to immediate use. For extended storage after reconstitution, we suggest the addition of an equal volume of glycerol to make a final glycerol concentration of 50% followed by storage at -20 °C. The concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol.
CD8 is expressed as either alpha/alpha homodimers or alpha/beta heterodimers on a subpopulation of T cells and NK cells.Synonyms: CD8B, CD8B1, T-cell surface glycoprotein CD8 beta chain