MHC, Class I 抗体
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北京 101111
Quick Overview for MHC, Class I 抗体 (ABIN181881)
抗原
See all MHC, Class I 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Affinity chromatography on Protein G
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免疫原
- Purified H-2Keb and H-2Deb MHC-I molecules. Spleen cells from immunised C1D mice were fused with cells of the X63 myeloma cell line.
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亚型
- IgG2b
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应用备注
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Immunoprecipitation. Flow Cytometry: 1/10 - 1/25, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.4, 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- MHC, Class I
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别名
- MHC Class I
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背景
- MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.Synonyms: HLA Class 1, MHC Class 1, Major Histocompatibility complex class I
抗原
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