CCL25 抗体 (Biotin)
Quick Overview for CCL25 抗体 (Biotin) (ABIN181760)
抗原
See all CCL25 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects TECK.
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纯化方法
- Affinity chromatography
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免疫原
- Highly pure (> 98 %) recombinant human TECK
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应用备注
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ELISA: Direct: To detect hTECK (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTECK. Sandwich: To detect hTECK (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanTECK as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hTECK. Western blot: To detect hTECK this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hTECK is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.
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缓冲液
- PBS, pH 7.2
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注意事项
- This product is photosensitive and should be protected from light. Should it contain a precipitate we recommend microcentrifugation before use.
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
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