IGF2 抗体 (Biotin)
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Quick Overview for IGF2 抗体 (Biotin) (ABIN181748)
抗原
See all IGF2 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects Insulin-like Growth Factor II.
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纯化方法
- Affinity chromatograophy
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免疫原
- Highly pure (> 98 %) recombinant human IGF-II
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应用备注
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ELISA: Direct: To detect hIGF-II (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-II. Sandwich: To detect hIGF-II (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanIGF-II as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hIGF-II. Western blot: To detect hIGF-II this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hIGF-II is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.
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缓冲液
- PBS, pH 7.2
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注意事项
- This product is photosensitive and should be protected from light
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
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- IGF2 (Insulin-Like Growth Factor 2 (IGF2))
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别名
- Insulin-Like Growth Factor II / IGF2
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背景
- The IGF2 gene encodes a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is expressed only from the paternally inherited allele and is an imprinted gene which is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3' region and to the upstream INS gene at the 5' region. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. Insulin Like Growth Factor-2 (IGF-2) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types.Synonyms: IGF-II, Somatomedin-A
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基因ID
- 3481
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NCBI登录号
- NP_000603
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UniProt
- P01344
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途径
- Hormone Activity, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Activated T Cell Proliferation
抗原
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