C19orf10 抗体 (Biotin)
Quick Overview for C19orf10 抗体 (Biotin) (ABIN181721)
抗原
See all C19orf10 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects SF-20.
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纯化方法
- Affinity chromatograophy
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免疫原
- Highly pure (> 98 %) recombinant murine SF-20
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应用备注
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ELISA: Direct: To detect mSF-20 by (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSF-20. Sandwich: To detect mSF-20 (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. This In conjunction with PolyclonalAnti-Murine SF-20 as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/wellof recombinant mSF-20. Western blot: To detect mSF-20 this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant mSF-20 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.
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缓冲液
- PBS, pH 7.2
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
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有效期
- 6 months
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- C19orf10 (Chromosome 19 Open Reading Frame 10 (C19orf10))
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别名
- SF20 / IL25
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背景
- Synonyms: C19orf10, IL-25, Interleukin-25, Stromal cell-derived growth factor SF20
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基因ID
- 28106
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NCBI登录号
- NP_543027
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UniProt
- Q9CPT4
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途径
- ER-Nucleus Signaling
抗原
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