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MIA2 抗体 (Biotin)

The 兔 多克隆 anti-MIA2 antibody is suitable to detect MIA2 in samples from 人. It has been validated for WB 和 EIA.
产品编号 ABIN181709
发货至: 中国
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for MIA2 抗体 (Biotin) (ABIN181709)

抗原

See all MIA2 抗体
MIA2 (Melanoma Inhibitory Activity Protein 2 (MIA2))

适用

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宿主

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克隆类型

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多克隆

标记

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This MIA2 antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), Enzyme Immunoassay (EIA)
  • 纯化方法

    Affinity chromatography

    免疫原

    Highly pure (> 98 %) recombinant human MIA-2
  • 应用备注

    ELISA: Direct: To detect hMIA-2 (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIA-2. Sandwich: To detect hMIA-2 (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanMIA-2 as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hMIA-2. Western blot: To detect hMIA-2 this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hMIA-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 -1 .0 mg/mL.

    缓冲液

    PBS, pH 7.2

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

    有效期

    6 months
  • 抗原

    MIA2 (Melanoma Inhibitory Activity Protein 2 (MIA2))

    别名

    MIA2

    背景

    MIA2 (Melanoma inhibitory activity 2) is a secreted cytokine and a member of the MIA/OTOR family. It is predominantly expressed in hepatocytes and may play a role in the pathophysiology of liver disease. Elevated levels of MIA2 may represent a clinically useful marker for diagnosis of hepatic disease activity and severity. MIA2 may act as a tumor suppressor in hepatocellular carcinoma.Synonyms: Melanoma inhibitory activity protein 2

    基因ID

    9606

    UniProt

    Q96PC5
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