MIA 抗体
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- 抗原 See all MIA 抗体
- MIA (Melanoma Inhibitory Activity (MIA))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This MIA antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- 特异性
- This antibody detects MIA.
- 纯化方法
- Immunoaffinity Chromatography.
- 免疫原
- Highly pure (> 98%) recombinant Human MIA
- Top Product
- Discover our top product MIA Primary Antibody
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anti-Melanoma Inhibitory Activity (MIA) (Internal Region) antibody
Verified MIA 适用: 人 ELISA, IHC, WB 宿主: 山羊 Polyclonal unconjugated
anti-Melanoma Inhibitory Activity (MIA) (AA 90-131) antibodyMIA 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Melanoma Inhibitory Activity (MIA) (AA 87-124) antibodyMIA 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Melanoma Inhibitory Activity (MIA) antibodyMIA 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Melanoma Inhibitory Activity (MIA) (AA 36-127) antibodyMIA 适用: 大鼠 IHC, WB, ICC 宿主: 兔 Polyclonal unconjugated
anti-Melanoma Inhibitory Activity (MIA) (C-Term) antibodyMIA 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Melanoma Inhibitory Activity (MIA) (AA 1-131) antibodyMIA 适用: 人 ELISA 宿主: 小鼠 Monoclonal 3A6 unconjugated
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- 应用备注
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Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sectionsof human pancreas infiltrating ductal adenocarcinoma tissue. The recommendedconcentration is 1.0-2.0 μg/mL with an overnight incubation at 4 °C. An HRP-labeledpolymer detecttion system was used with an alcohol-soluble AEC chromogen. Optimalresults for these conditions were achieved with heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer enzyme induced antigen retrievalwith proteinase K at roomtemperature.
Optimal conditions may vary. Tissue samples were provided by theCooperative Human Tissue Network which is funded by the National Cancer Institute. Indirect ELISA: To detect Human MIA (using 100 μL/well antibody solution) a concentrationof 0.5-2.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIA. Sandwich ELISA: To detect Human MIA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. In conjunction withBiotinylated Anti-Human MIA (ABIN181670 or ABIN181669) as a Detection antibody, itallows the detection of at least 0.2-0.4 ng/well of recombinant Human MIA. Western blot: To detect Human MIA this antibody can be used at a concentration of 0.1-0.2 - 限制
- 仅限研究用
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- 溶解方式
- Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
- 缓冲液
- PBS, pH 7.2 without preservatives.
- 储存液
- Without preservative
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
- 有效期
- 6 months
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- 抗原
- MIA (Melanoma Inhibitory Activity (MIA))
- 别名
- MIA (MIA 产品)
- 别名
- CD-RAP antibody, Mia1 antibody, Cdrap antibody, zgc:194157 antibody, mia antibody, CDRAP antibody, Fdp antibody, MIA antibody, MIAL antibody, melanoma inhibitory activity antibody, otoraplin antibody, cilia and flagella associated protein 100 antibody, MIA antibody, Mia antibody, mia antibody, Otor antibody, CFAP100 antibody
- 背景
- Melanoma Inhibitory Activity (MIA) was originally identified as an inhibitor of the in vitro growth of malignant melanoma cells. It was the first discovered member of a family of secreted cytokines termed the MIA/OTOR family. The four known members of this family, Melanoma Inhibitory Activity, MIA2, OTOR and TANGO each contain a Src homology-3 (SH3)-like domain. Melanoma Inhibitory Activity is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extracellular matrix in vivo. Elevated levels of Melanoma Inhibitory Activity may represent a clinically useful marker for diagnosis of melanoma metastasis as well as a potential marker for rheumatoid arthritis.Synonyms: Melanoma inhibitory activity, Melanoma-derived growth regulatory protein
- 基因ID
- 8190
- NCBI登录号
- NP_001189482
- UniProt
- Q16674
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