MIA 抗体
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北京 101111
Quick Overview for MIA 抗体 (ABIN181672)
抗原
See all MIA 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects MIA.
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纯化方法
- Immunoaffinity Chromatography.
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免疫原
- Highly pure (> 98%) recombinant Human MIA
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anti-Melanoma Inhibitory Activity (MIA) (Internal Region) antibody
Verified MIA 适用: 人 ELISA, IHC, WB 宿主: 山羊 Polyclonal unconjugated
anti-Melanoma Inhibitory Activity (MIA) antibodyMIA 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Melanoma Inhibitory Activity (MIA) (AA 87-124) antibodyMIA 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Melanoma Inhibitory Activity (MIA) antibodyMIA 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Melanoma Inhibitory Activity (MIA) (AA 36-127) antibodyMIA 适用: 大鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Melanoma Inhibitory Activity (MIA) (C-Term) antibodyMIA 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Melanoma Inhibitory Activity (MIA) (AA 1-131) antibodyMIA 适用: 人 ELISA 宿主: 小鼠 Monoclonal 3A6 unconjugated
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应用备注
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Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sectionsof human pancreas infiltrating ductal adenocarcinoma tissue. The recommendedconcentration is 1.0-2.0 μg/mL with an overnight incubation at 4 °C. An HRP-labeledpolymer detecttion system was used with an alcohol-soluble AEC chromogen. Optimalresults for these conditions were achieved with heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer enzyme induced antigen retrievalwith proteinase K at roomtemperature.
Optimal conditions may vary. Tissue samples were provided by theCooperative Human Tissue Network which is funded by the National Cancer Institute. Indirect ELISA: To detect Human MIA (using 100 μL/well antibody solution) a concentrationof 0.5-2.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIA. Sandwich ELISA: To detect Human MIA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. In conjunction withBiotinylated Anti-Human MIA (ABIN181670 or ABIN181669) as a Detection antibody, itallows the detection of at least 0.2-0.4 ng/well of recombinant Human MIA. Western blot: To detect Human MIA this antibody can be used at a concentration of 0.1-0.2 -
限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
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有效期
- 6 months
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- MIA (Melanoma Inhibitory Activity (MIA))
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别名
- MIA
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背景
- Melanoma Inhibitory Activity (MIA) was originally identified as an inhibitor of the in vitro growth of malignant melanoma cells. It was the first discovered member of a family of secreted cytokines termed the MIA/OTOR family. The four known members of this family, Melanoma Inhibitory Activity, MIA2, OTOR and TANGO each contain a Src homology-3 (SH3)-like domain. Melanoma Inhibitory Activity is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extracellular matrix in vivo. Elevated levels of Melanoma Inhibitory Activity may represent a clinically useful marker for diagnosis of melanoma metastasis as well as a potential marker for rheumatoid arthritis.Synonyms: Melanoma inhibitory activity, Melanoma-derived growth regulatory protein
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基因ID
- 8190
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NCBI登录号
- NP_001189482
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UniProt
- Q16674
抗原
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