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APOE 抗体 (Isoform E3) (Biotin)

This anti-APOE antibody is a 兔 多克隆 antibody detecting APOE in WB 和 EIA. Suitable for 人.
产品编号 ABIN181665
发货至: 中国

Quick Overview for APOE 抗体 (Isoform E3) (Biotin) (ABIN181665)

抗原

See all APOE 抗体
APOE (Apolipoprotein E (APOE))

适用

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宿主

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克隆类型

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多克隆

标记

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This APOE antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), Enzyme Immunoassay (EIA)
  • 抗原表位

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    Isoform E3

    特异性

    This antibody detects ApoE3. Cross reacts with Human ApoE2 and Human ApoE4 in both Sandwich ELISA and Western Blot.

    纯化方法

    Affinity Chromatography.

    免疫原

    E.Coli derived recombinant Human ApoE3.
  • 应用备注

    Direct ELISA: To detect Human ApoE3 by Direct ELISA (using 100 μL/well antibody solution)a concentration of approx. 1.0 μg/mL of this antibody is required. This Biotinylated polyclonalantibody allows the detection of at least 0.2-0.4 ng/well of recombinant Human ApoE3. Sandwich ELISA: To detect Human ApoE3 by Sandwich ELISA (using 100 μL/well) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This Biotinylated antibody, inconjunction with Affinity purified anti-Human ApoE3 (ABIN181667 or ABIN181668) as acapture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant HumanApoE3. Western blot: To detect Human ApoE3 this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant Human ApoE3 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

    缓冲液

    PBS, pH 7.2 without preservatives.

    储存液

    Without preservative

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

    有效期

    6 months
  • 抗原

    APOE (Apolipoprotein E (APOE))

    别名

    Apolipoprotein E (Apo E)

    背景

    Apolipoprotein E3 mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo E receptor (chylomicron remnant) of hepatic tissues.Synonyms: Apo-E, ApoE

    基因ID

    348

    NCBI登录号

    NP_000032

    UniProt

    P02649

    途径

    Regulation of Cell Size, Lipid Metabolism
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