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IL-2 抗体

IL2 适用: 人 WB, EIA, Func, IHC (p) 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN181648
发货至: 中国
  • 抗原 See all IL-2 (IL2) 抗体
    IL-2 (IL2) (Interleukin 2 (IL2))
    适用
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    山羊
    克隆类型
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    多克隆
    标记
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    This IL-2 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    纯化方法
    Immunoaffinity chromatography
    免疫原
    Highly pure (> 98 %) recombinant human IL-2
    Top Product
    Discover our top product IL2 Primary Antibody
  • 应用备注
    Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity ofhIL-2 (2.0 ng/mL), a concentration of 0.12 - 0.16 μg/mL of this antibody is required. ELISA: Indirect: To detect hIL-2 (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-2. Sandwich: To detect hIL-2 (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with Biotinylated Anti-HumanIL-2 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hIL-2. Western blot: To detect hIL-2 this antibody can be used at a concentration of 0.1 - 0.2g/mL. Used in conjunction with compatible secondary reagents the detection limit forrecombinant hIL-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 溶解方式
    Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.
    缓冲液
    PBS, pH 7.2
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
    有效期
    6 months
  • 抗原
    IL-2 (IL2) (Interleukin 2 (IL2))
    别名
    Interleukin-2 / IL2 (IL2 产品)
    别名
    IL2 antibody, IL-2 antibody, il2 antibody, TCGF antibody, lymphokine antibody, Il-2 antibody, POIL2 antibody, interleukin 2 antibody, interleukin-15 antibody, IL2 antibody, il2 antibody, LOC100303688 antibody, Il2 antibody
    背景
    Interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells.Synonyms: IL-2, T-cell growth factor, TCGF
    基因ID
    9606
    UniProt
    P60568
    途径
    JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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