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CD22 抗体

CD22 适用: 人 WB, EIA 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN181644
发货至: 中国
  • 抗原 See all CD22 抗体
    CD22 (CD22 Molecule (CD22))
    适用
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    宿主
    • 96
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    • 13
    克隆类型
    • 118
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    多克隆
    标记
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    This CD22 antibody is un-conjugated
    应用范围
    • 107
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    Western Blotting (WB), Enzyme Immunoassay (EIA)
    纯化方法
    Immunoaffinity chromatography
    免疫原
    Highly pure (> 98 %) recombinant human CD22
    Top Product
    Discover our top product CD22 Primary Antibody
  • 应用备注
    ELISA: Indirect: To detect hCD22 (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCD22. Sandwich: To detect hCD22 (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with Biotinylated Anti-HumanCD22 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hCD22. Western blot: To detect hCD22 this antibody can be used at a concentration of 0.1 - 0.2g/mL. Used in conjunction with compatible secondary reagents the detection limit forrecombinant hCD22 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 溶解方式
    Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.
    缓冲液
    PBS, pH 7.2
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
    有效期
    6 months
  • 抗原
    CD22 (CD22 Molecule (CD22))
    别名
    CD22 (CD22 产品)
    别名
    A530093D23 antibody, Lyb-8 antibody, Lyb8 antibody, SIGLEC-2 antibody, SIGLEC2 antibody, siglec-2 antibody, CD22 antigen antibody, CD22 molecule antibody, Cd22 antibody, CD22 antibody
    背景
    CD22 is a type 1 integral membrane glycoprotein with molecular weight of 130 to 140kD. It is expressed in both the cytoplasm and cell membrane of B lymphocytes. CD22 antigen appears early in B cell lymphocyte differentiation at approximately the same stage as the CD19 antigen. Unlike other B cell markers, CD22 membrane expression is limited to the late differentiation stages comprised between mature B cells (CD22+) and plasma cells (CD22-), and may thus prove useful in phenotyping mature leukemias. CD22 is also strongly expressed in hairy cell leukemia. There are two different isoforms of CD22. It exists predominantly as a monomer of the beta isoform but can also be found as a heterodimer composed of the beta isoform and a second, shorter isoform (CD22 alpha).Synonyms: B-cell receptor CD22, B-lymphocyte cell adhesion molecule, BL-CAM, Leu-14, SIGLEC2, Sialic acid-binding Ig-like lectin 2, Siglec-2
    基因ID
    9606
    UniProt
    P20273
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