RELM alpha 抗体 (Biotin)
Quick Overview for RELM alpha 抗体 (Biotin) (ABIN181629)
抗原
See all RELM alpha 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects RELM alpha.
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纯化方法
- Affinity chromatography
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免疫原
- Highly pure (> 98 %) recombinant murine RELMalpha
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应用备注
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ELISA: Direct: To detect mRELMα by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant mRELMα. Sandwich: To detect mRELMα by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withPolyclonal Anti-Murine RELMα as a capture antibody, it allows the detection of at least 0.2 -0.4 ng/well of recombinant mRELMα. Western Blot: To detect mRELMα by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mRELMα is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.
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缓冲液
- PBS, pH 7.2
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
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有效期
- 6 months
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