IL-19 抗体 (Biotin)
Quick Overview for IL-19 抗体 (Biotin) (ABIN181613)
抗原
See all IL-19 (IL19) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects Interleukin-19.
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纯化方法
- Affinity chromatography
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免疫原
- Highly pure (> 98 %) recombinant human IL-19
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应用备注
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ELISA: Direct: To detect hIL-19 by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hIL-19. Sandwich: To detect hIL-19 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withPolyclonal Anti-Human IL-19 as a capture antibody, it allows the detection of at least 0.2 -0.4 ng/well of recombinant hIL-19. Western Blot: To detect hIL-19 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-19 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.
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缓冲液
- PBS, pH 7.2
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
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有效期
- 6 months
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- IL-19 (IL19) (Interleukin 19 (IL19))
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别名
- Interleukin-19 / IL19
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背景
- IL19 is a cytokine that belongs to the IL10 cytokine subfamily. This cytokine is found to be preferentially expressed in monocytes. It can bind the IL20 receptor complex and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). A similar cytokine in mouse is reported to up-regulate the expression of IL6 and TNF-alpha and induce apoptosis, which suggests a role of this cytokine in inflammatory responses. Little is known about the biologic function and gene regulation of IL19. Alternatively spliced transcript variants encoding distinct isoforms have been described.Synonyms: IL-19, Melanoma differentiation-associated protein-like protein, NG.1, ZMDA
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基因ID
- 29949
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NCBI登录号
- NP_037503
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UniProt
- Q9UHD0
抗原
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