IL-15 抗体
Quick Overview for IL-15 抗体 (ABIN181596)
抗原
See all IL-15 (IL15) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects Mouse IL-15. Other species not tested.
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纯化方法
- Immunoaffinity chromatography
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免疫原
- Highly pure (> 98 %) recombinant murine IL-15.
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anti-Interleukin 15 (IL15) (AA 50-162) antibody
IL15 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 15 (IL15) (AA 49-162) antibodyIL15 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2F9 unconjugated
anti-Interleukin 15 (IL15) (AA 49-162) antibodyIL15 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 15 (IL15) (AA 48-162) antibodyIL15 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 15 (IL15) (AA 49-162) antibodyIL15 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 15 (IL15) (AA 1-162) antibodyIL15 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 15 (IL15) (AA 30-129) antibodyIL15 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1H6 unconjugated
anti-Interleukin 15 (IL15) (AA 49-162) antibodyIL15 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2F6 unconjugated
anti-Interleukin 15 (IL15) (AA 46-162) antibodyIL15 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2F9 unconjugated
anti-Interleukin 15 (IL15) antibodyIL15 适用: 人 WB, ELISA, IP, Func, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of IL-15(10.0 ng/mL), a concentration of 0.09 - 0.149 μg/mL is required. ELISA: Indirect: To detect IL-15 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. In conjunction with compatible secondary
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限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL. Centrifuge vial prior to opening.
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缓冲液
- PBS, pH 7.2
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
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有效期
- 6 months
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- IL-15 (IL15) (Interleukin 15 (IL15))
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别名
- Interleukin-15 / IL15
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背景
- IL15 (114 amino acids) is a cytokine that regulates T and natural killer cell activation and proliferation. It has a predicted molecular mass of approximately 12.5 kDa. Human IL15 shares approximately 97 % and 73 % amino acid sequence identity with simian and mouse IL15, respectively. Both human and simian IL15 are active on mouse cells. IL15 was initially isolated from the simian kidney epithelial cell line CV1/EBNA. It has also been isolated from mouse and human cell sources. The cytokines IL15 and IL2 share many biological properties and stimulatory activities (T, B, and NK cells). Both IL15 and IL2 stimulate mouse CTLL2 cells. In activated peripheral blood T lymphocytes, IL2 is highly expressed but the expression of IL15 is not detectable. There is no sequence homology between IL15 and IL2, though computer modeling indicates both possess a four alpha helical bundle structure. IL15 competes for binding sites with IL2, as both IL2 and IL15 stimulate the growth of cells through the IL2 receptor. IL15 mRNA is expressed in many cell types and tissues including adherent peripheral blood mononuclear cells, fibroblasts, and epithelial cells, monocytes, placenta, and skeletal muscle. IL-15 (14-15 kD) is a member of the four alpha-helical bundle family of cytokines. It is very similar to IL-2, except that IL-15 has an IL-15 alpha receptor subunit. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. This implies that IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases.Synonyms: IL-15
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基因ID
- 16168
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NCBI登录号
- NP_032383
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UniProt
- P48346
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途径
- JAK/STAT Signaling, Glycosaminoglycan Metabolic Process
抗原
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