CCL26 抗体
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北京 101111
Quick Overview for CCL26 抗体 (ABIN181588)
抗原
See all CCL26 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects Eotaxin-3.
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纯化方法
- Immunoaffinity chromatography
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免疫原
- Highly pure (> 98 %) recombinant human Eotaxin-3
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应用备注
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhEotaxin-3 (100 ng/mL), a concentration of 5.0 - 7.0 μg/mL of this antibody is required. ELISA: Indirect: To detect hEotaxin-3 by indirect ELISA (using 100 μL/well antibody solution)a concentration of 0.5 - 2.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hEotaxin-3. Sandwich: To detect hEotaxin-3 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. In conjunction with Biotinylated Anti-HumanEotaxin-3 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hEotaxin-3. Western Blot: To detect hEotaxin-3 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hEotaxin-3 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.
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缓冲液
- PBS, pH 7.2
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
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有效期
- 6 months
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- CCL26 (Chemokine (C-C Motif) Ligand 26 (CCL26))
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别名
- Eotaxin-3 / CCL26
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背景
- Eotaxin 3 is a recently discovered CC chemokine. It binds to the CCR3 receptor and is highly chemotactic for eosinophils and basophils.Synonyms: C-C motif chemokine 26, CC chemokine IMAC, IMAC, MIP4 alpha, Macrophage inflammatory protein 4-alpha, SCYA26, Small-inducible cytokine A26, TSC-1, TSC1, Thymic stroma chemokine-1
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基因ID
- 10344
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NCBI登录号
- NP_006063
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UniProt
- Q9Y258
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途径
- Regulation of Actin Filament Polymerization
抗原
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