FGF17
适用: 人
IF (cc), IF (p)
宿主: 兔
Polyclonal
Alexa Fluor 350
应用备注
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhFGF-17 (1.5 ng/mL), a concentration of 0.020 - 0.035 μg/mL of this antibody is required. ELISA: Indirect: To detect hFGF-17 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-17. Sandwich: To detect hFGF-17 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. This In conjunction with BiotinylatedAnti-Human FGF-17 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-17. Western Blot: To detect hFGF-17 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hFGF-17 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.
缓冲液
PBS, pH 7.2
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
储存方法
Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
FGF17 belongs to the heparin-binding growth factors family. It may be a signaling molecule in the induction and patterning of the embryonic brain and is preferentially expressed in the embryonic brain. There are two named isoforms.Synonyms: FGF-17, Fibroblast growth factor 17