CCL8 抗体 (Biotin)
Quick Overview for CCL8 抗体 (Biotin) (ABIN181573)
抗原
See all CCL8 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects Macrophage Chemotactic Protein-2.
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纯化方法
- Affinity chromatography
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免疫原
- Highly pure (> 98 %) recombinant mouse MCP-2
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anti-Chemokine (C-C Motif) Ligand 8 (CCL8) (AA 24-99) antibody
CCL8 适用: 人 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 8 (CCL8) (AA 1-99) antibodyCCL8 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 8 (CCL8) (AA 20-94) antibodyCCL8 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 8 (CCL8) (AA 1-99) antibodyCCL8 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 8 (CCL8) (AA 24-99) antibodyCCL8 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 8 (CCL8) (C-Term) antibodyCCL8 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 8 (CCL8) (AA 23-99) antibodyCCL8 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: Direct: To detect mMCP-2 by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-2. Sandwich: To detect mMCP-2 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withPolyclonal Anti-Murine MCP-2 as a capture antibody, it allows the detection of at least 0.2 -0.4 ng/well of recombinant mMCP-2. Western Blot: To detect mMCP-2 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mMCP-2 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.
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缓冲液
- PBS, pH 7.2
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
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有效期
- 6 months
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- CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))
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别名
- MCP2 / CCL8
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背景
- MCP 2 (Monocyte Chemoattractant Protein 2) is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. MCP 2 is important to inflammatory host responses, and is found in the highest concentration in the small intestine and peripheral blood cells. This protein is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection.Synonyms: C-C motif chemokine 8, CCL-8, HC14, MCP-2, Monocyte Chemotactic Protein-2, Monocyte chemoattractant protein 2, SCYA10, SCYA8, Small-inducible cytokine A8
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基因ID
- 100503254
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NCBI登录号
- NP_067418
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UniProt
- Q9Z121
抗原
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