CXCL7 抗体 (Biotin)
Quick Overview for CXCL7 抗体 (Biotin) (ABIN181561)
抗原
See all CXCL7 (PPBP) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects Neutrophil Activating Protein-2.
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纯化方法
- Affinity chromatography
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免疫原
- Highly pure (> 98 %) recombinant human NAP-2
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应用备注
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ELISA: Direct: To detect hNAP-2 by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. In conjunction with compatible secondaryreagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNAP-2. Sandwich: To detect hNAP-2 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withPolyclonal Anti-Human NAP-2 as a capture antibody, it allows the detection of at least 0.2 -0.4 ng/well of recombinant hNAP-2. Western Blot: To detect hNAP-2 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hNAP-2 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.
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缓冲液
- PBS, pH 7.2
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
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有效期
- 6 months
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- CXCL7 (PPBP) (Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP))
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别名
- NAP2 / PPBP / CXCL7
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背景
- NAP2 is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and sythesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.Synonyms: C-X-C motif chemokine 7, CTAP3, Leukocyte-derived growth factor, Macrophage-derived growth factor, SCYB7, Small-inducible cytokine B7, TGB1, THBGB1
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基因ID
- 5473
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NCBI登录号
- NP_002695
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UniProt
- P02775
抗原
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