Persephin 抗体 (Biotin)
Quick Overview for Persephin 抗体 (Biotin) (ABIN181557)
抗原
See all Persephin (PSPN) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
 - This antibody detects Persephin.
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纯化方法
 - Affinity chromatography
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免疫原
 - Highly pure (> 98 %) recombinant human Persephin
 
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应用备注
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                        ELISA: Direct: To detect hPersephin by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hPersephin. Sandwich: To detect hPersephin by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. This In conjunction with PolyclonalAnti-Human Persephin as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPersephin. Western Blot: To detect hPersephin by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hPersephin is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions. 
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 
                                            
限制
 - 仅限研究用
 
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溶解方式
 - Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.
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缓冲液
 - PBS, pH 7.2
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注意事项
 - Avoid repeated freezing and thawing.
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储存条件
 - -20 °C
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储存方法
 - Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
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有效期
 - 6 months
 
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