Follistatin 抗体
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北京 101111
Quick Overview for Follistatin 抗体 (ABIN181535)
抗原
See all Follistatin (FST) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detetcs Follistatin.
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纯化方法
- Affinity purified
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免疫原
- Highly pure (> 98 %) recombinant human Follistatin
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anti-Follistatin (FST) (AA 151-250) antibody
FST 适用: 人 ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Follistatin (FST) (AA 35-283) antibodyFST 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Follistatin (FST) (AA 312-340) antibodyFST 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Follistatin (FST) (AA 1-344) antibodyFST 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1G4 unconjugated
anti-Follistatin (FST) (AA 1-344) antibodyFST 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4B11 unconjugated
anti-Follistatin (FST) (Internal Region) antibodyFST 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Follistatin (FST) antibodyMicroarray verified FST 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB899576 unconjugated
anti-Follistatin (FST) antibodyMicroarray verified FST 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB604608 unconjugated
anti-Follistatin (FST) antibodyMicroarray verified FST 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB79927 unconjugated
anti-Follistatin (FST) antibodyFST 适用: 人 ELISA, IHC (f) 宿主: 小鼠 Monoclonal unconjugated
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应用备注
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ELISA: Direct: To detect hFollistatin by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFollistatin. Sandwich: To detect hFollistatin by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. In conjunction with Polyclonal Anti-HumanFollistatin as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hFollistatin. Western Blot: To detect hFollistatin by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hFollistatin is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.
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缓冲液
- PBS, pH 7.2
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
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有效期
- 6 months
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- Follistatin (FST)
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别名
- Follistatin
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背景
- Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. There is evidence linking polycystic ovary syndrome and follistatin. Follistatin binds directly to activin and functions as an activin antagonist. It specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone. There are three different isoforms, generated by alternative splicing.Synonyms: Activin-binding protein, FS, FST
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基因ID
- 10468
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NCBI登录号
- NP_006341
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UniProt
- P19883
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途径
- Negative Regulation of Hormone Secretion
抗原
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