ELISA: In a sandwich ELISA (assuming 100μL/well), a concentration of 4.0-8.0 μg/mL of thisantibody will detect at least 200 pg/mL of recombinant human Resistin when used withBiotin anti-human Resistin antibody (cat. PP1214B) as the detection antibody at aconcentration of at least 0.5-1.0 μg/mL. Western Blot: To detect Human Resistin by Western Blot analysis this antibody can beused at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatiblesecondary reagents the detection limit for recombinant hResistin is 0.20-0.40 ng/lane,under non-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore in sterile water to a concentration of 1.0 mg/mL
缓冲液
PBS without preservatives
储存液
Without preservative
储存条件
4 °C/-20 °C
储存方法
The lyophilized antibody is stable at room temperature for one month or at -20 °C forlonger. Following reconstitution it is stable at 2-8 °C for six weeks. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Resistin, also known as Adipocyte Secreted Factor (ADSF) and FIZZ3, is a cytokine that is specifically secreted by adipocytes and seems to suppress the ability of insulin to stimulate glucose uptake into adipose cells. While the function of resistin is not well understood, it appears to have key paracrine roles and has a suggested role in type 2 diabetes.Synonyms: ADSF, Adipose tissue-specific secretory factor, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, Cysteine-rich secreted protein FIZZ3, FIZZ3, HXCP1, RETN, RSTN