IL11
适用: 小鼠, 大鼠
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
AbBy Fluor® 488
应用备注
Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity ofhIL-11 (5.0 ng/mL), a concentration of 2.4-3.6 μg/mL is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 8-9 μg/mL will detectat least 0.2 ng/well of recombinant Human IL-11 when used with Biotin anti-Human IL-11antibody (cat. PP1033B) as the detection antibody at a concentration of at least 2 μg/mL. Western Blot: To detect hIL-11 by Western Blot analysis this antibody can be used at aconcentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-11 is 0.25-0.50 ng/lane, under non-reducingconditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore in sterile water to a concentration of 1.0 mg/mL.
缓冲液
PBS without preservatives
储存液
Without preservative
储存条件
4 °C/-20 °C
储存方法
The lyophilized antibody is stable at room temperature for one month or at -20 °C forlonger. Following reconstitution it is stable at 2 - 8 °C for six weeks. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Interleukin 11 is a pleiotropic cytokine produced by mesenchymal-derived adherent cells. IL11 shares many functions of IL6 and LIF, including potentiation of megakaryocyte activity, enhancement of human myeloma cell proliferation, and enhancement of hepatic acute phase protein production. Interleukin 11 directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells, and induces megakaryocyte maturation resulting in increased platelet production. Clinically it is used to prevent severe thrombocytopenia and the reduction of the need for platelet transfusion following myelosuppressive chemotherapy.Synonyms: AGIF, Adipogenesis inhibitory factor, IL-11