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PRNP 抗体

Name: PRNP 抗体
SKU: ABIN180947
Availability: OutOfStock

This anti-PRNP antibody is a 小鼠单克隆 antibody detecting PRNP in IHC (p) 和 EIA. Suitable for 绵羊. This Primary Antibody has been cited in 4+ publications.

Rat brain lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-Prion Protein, clone 2G11 (1 µg/mL). (PRNP 抗体)

Immunohistochemistry: 1:50-1:300 working dilutions of anti-Prion Protein, clone 2G11 localizes prion protein in rat brain paraffin embedded tissue (High & Low Magnification). (PRNP 抗体)

IHC (p)

2 images

(4 references)

产品编号 ABIN180947

发货至: 中国

Quick Overview for PRNP 抗体 (ABIN180947)

抗原

See all PRNP 抗体

PRNP (Prion Protein (PRNP))

适用

绵羊

宿主

小鼠

克隆类型

单克隆

标记

This PRNP antibody is un-conjugated

应用范围

Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

克隆位点

2G11

Discover our top product PRNP Primary Antibody

特异性

This antibody recognises the scrapie-associated protease-resistant isoform (PrPsc) of ovine prion protein (PrP). Clone 2G11 specifically recognises the Re151-Re159 sequence and does not recognise the non-pathogenic PrPec form.

产品特性

Synonyms: Major prion protein, PrP27-30, PrP33-35C, ASCR, PRNP, PRIP

纯化方法

Affinity chromatography on Protein G

免疫原

Synthetic peptide 146-Re154Re171-182 of ovine PrP

亚型

IgG2a
anti-Prion Protein (PRNP) (AA 23-230) antibody
PRNP 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Prion Protein (PRNP) (AA 23-230) antibody
PRNP 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 3G10G9 unconjugated

anti-Prion Protein (PRNP) antibody
PRNP 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Prion Protein (PRNP) antibody
PRNP 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Prion Protein (PRNP) (AA 23-230) antibody
PRNP 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Prion Protein (PRNP) (AA 23-64) antibody
PRNP 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Prion Protein (PRNP) antibody
PRNP 适用: 人, 大鼠, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Prion Protein (PRNP) antibody
PRNP 适用: 人, Hamster WB, ELISA, IHC, IP 宿主: 小鼠 Monoclonal 3F4 unconjugated Recombinant Antibody

anti-Prion Protein (PRNP) (Prion) antibody
PRNP 适用: 人 WB 宿主: 小鼠 Monoclonal EM-20 unconjugated

anti-Prion Protein (PRNP) (AA 143-230) antibody
PRNP 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

ELISA: 1/50 - 1/1000, Tested on peptide. Immunohistochemistry on paraffin sections: suitable for use on tissue fixed in 4 % neutralbuffered formalin. Treatment of tissue sections in 98 % formic acid, for 30 minutes, isrecommended prior to pre-treatment with trypsin at 37C for 5 minutes followed by heatmediated retrieval with 10 mM citrate buffer pH 6.1.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

1.0 mg/mL

缓冲液

PBS, pH 7.4 containing 0.09 % Sodium Azide

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C/-20 °C

储存方法

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

有效期

12 months
Reil, Bux: "Geno- and phenotyping of human neutrophil antigens." in: Methods in molecular biology (Clifton, N.J.), Vol. 1310, pp. 193-203, (2016) (PubMed).

Lederle, Hartenstein, Meides, Kunzelmann, Werb, Angel, Mueller: "MMP13 as a stromal mediator in controlling persistent angiogenesis in skin carcinoma." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1175-84, (2010) (PubMed).

Rolhion, Barnich, Bringer, Glasser, Ranc, Hébuterne, Hofman, Darfeuille-Michaud: "Abnormally expressed ER stress response chaperone Gp96 in CD favours adherent-invasive Escherichia coli invasion." in: Gut, Vol. 59, Issue 10, pp. 1355-62, (2010) (PubMed).

Andréoletti, Berthon, Levavasseur, Marc, Lantier, Monks, Elsen, Schelcher: "Phenotyping of protein-prion (PrPsc)-accumulating cells in lymphoid and neural tissues of naturally scrapie-affected sheep by double-labeling immunohistochemistry." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 50, Issue 10, pp. 1357-70, (2002) (PubMed).
抗原

PRNP (Prion Protein (PRNP))

别名

CD230 / PrP

背景

The prion protein is encoded by the PrP gene and is constitutively expressed as the normal cellular isoform PrPec by many cell types. Transmissible spongiform encephalopathies (TSE) are neurodegenerative diseases in which the accumulation of the abnormal PrPesc isoform in tissues, of the lymphoid and central nervous system, is thought to be a key event. Studies suggest that PrPesc is the causative agent of transmissible spongiform encephalitis (TSE) but this has not been confirmed.Synonyms: ASCR, Major prion protein, PRIP, PRNP, PrP27-30, PrP33-35C

基因ID

493887

UniProt

P23907

途径

Transition Metal Ion Homeostasis, Activated T Cell Proliferation