NCK1 抗体
(1 reference)Quick Overview for NCK1 抗体 (ABIN1774727)
抗原
See all NCK1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-NCK1 Purified
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特异性
- The mouse monoclonal antibody EM-06 recognizes NCK1 (NCK alpha), an ubiquitously expressed cytoplasmic SH2/SH3 adaptor protein important for organization of actin cytoskeleton structures.
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交叉反应 （详细）
- Human, Mouse
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Purified recombinant second SH3 domain of human Nck1 fused to GST
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亚型
- IgG1
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anti-NCK Adaptor Protein 1 (NCK1) antibody
NCK1 适用: 人, 猴 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5B7 unconjugated
anti-NCK Adaptor Protein 1 (NCK1) (AA 185-294) antibodyNCK1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A1 unconjugated
anti-NCK Adaptor Protein 1 (NCK1) antibodyNCK1 适用: 人, 猴 WB, FACS, IHC (p), EIA 宿主: 小鼠 Monoclonal 5B7 unconjugated
anti-NCK Adaptor Protein 1 (NCK1) (AA 58-377) antibodyNCK1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-NCK Adaptor Protein 1 (NCK1) antibodyNCK1 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-NCK Adaptor Protein 1 (NCK1) (AA 1-377) antibodyNCK1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-NCK Adaptor Protein 1 (NCK1) (AA 53-333) antibodyNCK1 适用: 人 WB, IHC, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-NCK Adaptor Protein 1 (NCK1) (AA 1-377) antibodyNCK1 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-NCK Adaptor Protein 1 (NCK1) (Internal Region) antibodyNCK1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-NCK Adaptor Protein 1 (NCK1) (AA 2-377) antibodyNCK1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blotting: Recommended dilution: 2 μg/mL, positive control: Jurkat cell lysate.
Immunocytochemistry: Recommended dilution: 2 μg/mL, positive control: human NCK1-transfected COS-7 cells. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze. Do not use after expiration date stamped on vial label.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "Nck recruitment to the TCR required for ZAP70 activation during thymic development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 190, Issue 3, pp. 1103-12, (2013) (PubMed).
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: "Nck recruitment to the TCR required for ZAP70 activation during thymic development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 190, Issue 3, pp. 1103-12, (2013) (PubMed).
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- NCK1 (NCK Adaptor Protein 1 (NCK1))
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别名
- NCK1
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背景
- NCK adaptor protein 1,NCK1 (NCK alpha) is a cytoplasmic adaptor protein that plays a universal role in coordinating the signaling networks critical for organizing the actin cytoskeleton, cell movement, or axon guidance, connecting transmembrane receptors to multiple intracellular signaling pathways. It contains one SH2 domain, through which NCK1 binds to phosphorylated domains of transmembrane signaling moleculs or certain adaptor proteins, and three SH3 domains for binding proline-rich sequences of other molecules involved in the process of nucleation and polymerization of the actin cytoskeleton.,NCK-1, NCKalpha
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基因ID
- 4690
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UniProt
- P16333
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途径
- TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
抗原
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