Profilin 抗体 (AA 42-47)
Quick Overview for Profilin 抗体 (AA 42-47) (ABIN1742560)
抗原
See all Profilin (PFN) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 42-47
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特异性
- Specific for birch profilin.
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纯化方法
- purified IgG
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免疫原
- Recombinant birch profilin. Epitope PQFKPQ (aa 42-47)
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亚型
- IgG2a
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应用备注
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WB: 1 : 500 up to 1 : 10000
IHC: not tested yet -
说明
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This antibody has been used successfully with eucaryotic and procaryotic expression vectors using the epitope as a tag (Rudiger et al. 1997).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
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缓冲液
- PBS
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注意事项
- Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
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储存条件
- -20 °C
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储存方法
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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: "Molecular and structural analysis of a continuous birch profilin epitope defined by a monoclonal antibody." in: The Journal of biological chemistry, Vol. 271, Issue 47, pp. 29915-21, (1997) (PubMed).
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: "Molecular and structural analysis of a continuous birch profilin epitope defined by a monoclonal antibody." in: The Journal of biological chemistry, Vol. 271, Issue 47, pp. 29915-21, (1997) (PubMed).
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- Profilin (PFN)
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别名
- Profilin
抗原
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