Profilin 抗体
This anti-Profilin antibody is a 小鼠 单克隆 antibody detecting Profilin in WB, ICC 和 IHC. Suitable for 人, Cow, 豚鼠 和 兔. This Primary Antibody has been cited in 6+ publications.
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(6 references)Quick Overview for Profilin 抗体 (ABIN1742559)
抗原
See all Profilin (PFN) 抗体
Profilin (PFN)
适用
人, Cow, 豚鼠, 兔
宿主
小鼠
克隆类型
单克隆
标记
This Profilin antibody is un-conjugated
应用范围
Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
克隆位点
2H11
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特异性
- Specific for profilin 1.
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纯化方法
- purified IgG
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免疫原
- Fusion protein of alpha-actinin from dictyostelium and bovine profilin.
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亚型
- IgG1
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anti-Profilin (PFN) (AA 1-133) antibody
PFN 适用: Mercenaria (Clam) ELISA, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB: 1 : 500 up to 1 : 5000 (AP staining)
IP: not tested yet -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
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缓冲液
- PBS
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注意事项
- Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
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储存条件
- -20 °C
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储存方法
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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: "Actin filaments at the leading edge of cancer cells are characterized by a high mobile fraction and turnover regulation by profilin I." in: PLoS ONE, Vol. 9, Issue 1, pp. e85817, (2014) (PubMed).
: "Complex formation between the postsynaptic scaffolding protein gephyrin, profilin, and Mena: a possible link to the microfilament system." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 23, Issue 23, pp. 8330-9, (2003) (PubMed).
: "A role for polyproline motifs in the spinal muscular atrophy protein SMN. Profilins bind to and colocalize with smn in nuclear gems." in: The Journal of biological chemistry, Vol. 274, Issue 53, pp. 37908-14, (2000) (PubMed).
: "Suppression of tumorigenicity in breast cancer cells by the microfilament protein profilin 1." in: The Journal of experimental medicine, Vol. 191, Issue 10, pp. 1675-86, (2000) (PubMed).
: "Effects of single amino acid substitutions in the actin-binding site on the biological activity of bovine profilin I." in: Journal of cell science, Vol. 111 ( Pt 22), pp. 3261-73, (1999) (PubMed).
: "Molecular and structural analysis of a continuous birch profilin epitope defined by a monoclonal antibody." in: The Journal of biological chemistry, Vol. 271, Issue 47, pp. 29915-21, (1997) (PubMed).
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: "Actin filaments at the leading edge of cancer cells are characterized by a high mobile fraction and turnover regulation by profilin I." in: PLoS ONE, Vol. 9, Issue 1, pp. e85817, (2014) (PubMed).
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- Profilin (PFN)
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别名
- Profilin
抗原
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