Profilin 抗体 (AA 1-140)
This anti-Profilin antibody is a 小鼠 单克隆 antibody detecting Profilin in WB, IHC 和 IHC (p). Suitable for 人, Cow, 豚鼠, Opossum 和 兔. This Primary Antibody has been cited in 6+ publications.
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(6 references)Quick Overview for Profilin 抗体 (AA 1-140) (ABIN1742559)
抗原
See all Profilin (PFN) 抗体
Profilin (PFN)
适用
人, Cow, 豚鼠, Opossum, 兔
宿主
小鼠
克隆类型
单克隆
标记
This Profilin antibody is un-conjugated
应用范围
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
克隆位点
2H11
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抗原表位
- AA 1-140
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原理
- Mouse anti-Bovine Profilin IgG
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特异性
- Specific for profilin 1.
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交叉反应 （详细）
- Reacts with: human (P07737), cow, rabbit, Guinea pig, opossum. No signal: mouse (P62962). Other species not tested yet.
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纯化方法
- purified IgG
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免疫原
- Recombinant protein corresponding to AA 1 to 140 from bovine Profilin1 (UniProt Id: P02584)
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亚型
- IgG1
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anti-Profilin (PFN) (AA 1-133) antibody
PFN 适用: Mercenaria (Clam) ELISA, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB:1 : 500 up to 1 : 5000 IHC:yes IHC-P:1 : 1000,
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.
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缓冲液
- PBS.
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注意事项
- Do not freeze! Antibodies should be stored at +4°C when still lyophilized.
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储存条件
- -20 °C,-80 °C,4 °C
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储存方法
- Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Do not freeze!
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: "Actin filaments at the leading edge of cancer cells are characterized by a high mobile fraction and turnover regulation by profilin I." in: PLoS ONE, Vol. 9, Issue 1, pp. e85817, (2014) (PubMed).
: "Complex formation between the postsynaptic scaffolding protein gephyrin, profilin, and Mena: a possible link to the microfilament system." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 23, Issue 23, pp. 8330-9, (2003) (PubMed).
: "A role for polyproline motifs in the spinal muscular atrophy protein SMN. Profilins bind to and colocalize with smn in nuclear gems." in: The Journal of biological chemistry, Vol. 274, Issue 53, pp. 37908-14, (2000) (PubMed).
: "Suppression of tumorigenicity in breast cancer cells by the microfilament protein profilin 1." in: The Journal of experimental medicine, Vol. 191, Issue 10, pp. 1675-86, (2000) (PubMed).
: "Effects of single amino acid substitutions in the actin-binding site on the biological activity of bovine profilin I." in: Journal of cell science, Vol. 111 ( Pt 22), pp. 3261-73, (1999) (PubMed).
: "Molecular and structural analysis of a continuous birch profilin epitope defined by a monoclonal antibody." in: The Journal of biological chemistry, Vol. 271, Issue 47, pp. 29915-21, (1997) (PubMed).
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: "Actin filaments at the leading edge of cancer cells are characterized by a high mobile fraction and turnover regulation by profilin I." in: PLoS ONE, Vol. 9, Issue 1, pp. e85817, (2014) (PubMed).
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- Profilin (PFN)
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别名
- Profilin
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背景
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Synonyms: profilin
Antigen Information: Profilins are regulators of the microfilament network
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UniProt
- P02584
抗原
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