TUBB3 抗体 (AA 443-450)
(1 reference)
(1 validation)Quick Overview for TUBB3 抗体 (AA 443-450) (ABIN1742553)
抗原
See all TUBB3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 443-450
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特异性
- Specific for beta3-tubulin.
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纯化方法
- antiserum
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免疫原
- Synthetic peptide (aa 443-450 of mouse beta3-tubulin) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine.
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anti-Tubulin, beta 3 (TUBB3) (N-Term) antibody
TUBB3 适用: 人, 小鼠, 大鼠, 犬, Pig WB, IHC (p), ICC, ICFC 宿主: 小鼠 Monoclonal TU-20 unconjugated
anti-Tubulin, beta 3 (TUBB3) (N-Term) antibody (FITC)TUBB3 适用: 人, 小鼠, 大鼠, 犬, Pig FACS, ICC 宿主: 小鼠 Monoclonal TU-20 FITC
anti-Tubulin, beta 3 (TUBB3) antibodyTUBB3 适用: 人 WB, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-Tubulin, beta 3 (TUBB3) antibodyTUBB3 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 8E3A7 unconjugated
anti-Tubulin, beta 3 (TUBB3) (AA 36-63) antibodyTUBB3 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB21098 unconjugated
anti-Tubulin, beta 3 (TUBB3) (AA 36-63) antibodyTUBB3 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, beta 3 (TUBB3) antibodyTUBB3 适用: 人, 小鼠, 大鼠 ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 2E9 unconjugated
anti-Tubulin, beta 3 (TUBB3) (AA 401-450) antibodyTUBB3 适用: 人, 小鼠, 大鼠, 犬 WB, ELISA, FACS, IHC (p), ICC, IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, beta 3 (TUBB3) antibodyTUBB3 适用: 人 WB, IHC, ELISA, IF, IP 宿主: 小鼠 Monoclonal unconjugated
anti-Tubulin, beta 3 (TUBB3) antibodyTUBB3 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal unconjugated
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应用备注
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WB: 1 : 1000 up to 1 : 10000 (AP staining)
ICC: 1 : 1000 up to 1 : 5000
IHC: 1 : 200 up to 1 : 500 -
限制
- 仅限研究用
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- by
- Kinexus Bioinformatics Corporation
- No.
- #029822
- 日期
- 2014.10.28
- 抗原
- Lot Number
- 302302/3
- Method validated
- Western Blotting
- Positive Control
- Mouse brain
- Negative Control
- Mouse heart, HeLa cells, A431 cells
- Notes
- A band was observed in the positive controls at the expected size (~50 kDa), which is lower in the negative controls.
- Primary Antibody
- Antigen: Tubulin, Beta, 3 (TUBB3) (AA 443-450)
- Catalog number: ABIN1742553
- Lot number: 302302/3
- Dilution: 1:1000
- Secondary Antibody
- Antibody: Donkey anti-Rabbit IgG Antibody (HRP)
- Lot number: F0613
- Dilution: 1:10,000
- Full Protocol
- 1. Cell/tissue total protein lysates were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 minutes prior to loading.
- 2. 15 μg of boiled lysate were loaded and resolved on a 12% SDS-polyacrylamide gel.
- 3. The Precision Plus Protein™ All Blue Prestained Standards from BioRad (161-0373) were used as molecular mass markers.
- 4. Proteins were transferred onto nitrocellulose membrane by tank transfer and protein transfer was confirmed with Ponceau S staining.
- 5. The immunoblot membrane was blocked in 2.5% skim milk and 1.5% BSA solution in TTBS at room temperature for 60 minutes.
- 6. The membrane was washed in TTBS twice for 5 minutes each.
- 7. The membrane was immersed with the protein side up in the antibody solution in TBS and incubated overnight at 4°C with gentle agitation.
- 8. The membrane was rinsed twice with TTBS.
- 9. The membrane was washed in TTBS twice for 5 minutes each.
- 10. The membrane was washed in TTBS once for 15 minutes.
- 11. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBS for 60 minutes at room temperature with gentle agitation.
- 12. The membrane was rinsed twice with TTBS.
- 13. The membrane was washed in TTBS twice for 5 minutes each.
- 14. The membrane was washed in TTBS once for 15 minutes.
- 15. Signals were detected by chemiluminescence (ECL). The blot was scanned for 320 seconds.
- 16. The membrane was rinsed three times with TTBS.
- 17. Repeated Steps 4-15 with the loading control antibody and its matching secondary antibody. The blot was scanned for 160 seconds.
- Experimental Notes
- - No experimental challenges noted.
生效 #029822 (Western Blotting)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Figure 1: Western Blot for Tubulin, Beta, 3 (TUBB3). Grey arrowhead indicates the expected molecular weight of ~50 kDa.]()
Figure 1: Western Blot for Tubulin, Beta, 3 (TUBB3). Grey arrowhead indicates the expected molecular weight of ~50 kDa.
Full Methods -
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状态
- Liquid
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缓冲液
- PBS
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注意事项
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Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable. -
储存条件
- 4 °C/-20 °C
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储存方法
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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: "Neuron-astrocyte interaction enhance GABAergic synaptic transmission in a manner dependent on key metabolic enzymes." in: Frontiers in cellular neuroscience, Vol. 9, pp. 120, (2015) (PubMed).
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: "Neuron-astrocyte interaction enhance GABAergic synaptic transmission in a manner dependent on key metabolic enzymes." in: Frontiers in cellular neuroscience, Vol. 9, pp. 120, (2015) (PubMed).
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- TUBB3 (Tubulin, beta 3 (TUBB3))
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别名
- beta3-Tubulin (TUBB3)
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背景
- Synonyms: TuJ1
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途径
- Microtubule Dynamics, M Phase
抗原
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