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alpha Tubulin 抗体 (AA 443-449)

This anti-alpha Tubulin antibody is a 兔 多克隆 antibody detecting alpha Tubulin in WB, ELISA, IHC 和 ICC. Suitable for 人, 小鼠, 大鼠, 小鸡 和 哺乳动物. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN1742550
发货至: 中国

Quick Overview for alpha Tubulin 抗体 (AA 443-449) (ABIN1742550)

抗原

See all alpha Tubulin (TUBA1) 抗体
alpha Tubulin (TUBA1)

适用

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人, 小鼠, 大鼠, 小鸡, 哺乳动物

宿主

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克隆类型

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多克隆

标记

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This alpha Tubulin antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)
  • 抗原表位

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    AA 443-449

    特异性

    Specific for alpha-tubulin (glu- and tyr-alpha-tubulin).

    纯化方法

    Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

    免疫原

    Synthetic peptide EGEEEGE (aa 443-449 of rat alpha-tubulin) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine.
  • 应用备注

    WB: 1 : 1000 up to 1 : 5000 (AP staining)
    IP: not tested yet
    ICC: 1 : 100 up to 1 : 1000
    IHC: 1 : 400

    说明

    ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742547 or ABIN1742549 as capture antibodies (protocol for sandwich-ELISA).

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

    缓冲液

    PBS

    注意事项

    Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

    储存条件

    -20 °C

    储存方法

    Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
  • Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

  • 抗原

    alpha Tubulin (TUBA1)

    别名

    alpha-Tubulin

    途径

    Microtubule Dynamics
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