Glu-alpha-Tubulin (C-Term) 抗体
This anti- antibody is a 小鼠 单克隆 antibody detecting in ELISA, IHC, WB 和 ICC. Suitable for 人, 小鼠, 大鼠, Eukaryotes 和 斑马鱼. This Primary Antibody has been cited in 9+ publications.
(9 references)Quick Overview for Glu-alpha-Tubulin (C-Term) 抗体 (ABIN1742545)
抗原
Glu-alpha-Tubulin
适用
人, 小鼠, 大鼠, Eukaryotes, 斑马鱼
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC)
克隆位点
1D5
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抗原表位
- C-Term
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特异性
- Specific for detyrosinated alpha-tubulin (glu-tubulin) and polyglutamylated tubulin (also beta-tubulin).
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交叉反应 （详细）
- No cross reaction to tyrosinated tubulin.
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免疫原
- Synthetic peptide VDSVEGEGEEEGEE (14 C-terminal aa of detyrosinated alpha-tubulin) coupled to BSA via glutaraldehyde. Epitope X-EE including the terminal alpha-carboxylate group. All linkages in the peptide involve alpha-peptide bonds.
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亚型
- IgG1
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应用备注
- WB: 1:1000, ICC: 1:200 up to 1:500, IHC: 1:500, IHC-P: 1:1000,
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说明
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WB: Tween 20 (other detergents not yet tested) should not be added to the blocking and washing solution. It greatly diminishes the intensity of the signal.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 100µl H2O to get a 1mg/ml solution in PBS.
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缓冲液
- PBS. Albumin and azide were added for stabilization.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze! Antibodies should be stored at +4°C when still lyophilized.
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储存条件
- -20 °C
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储存方法
- Reconstitute and aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized.
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: "Tubulin detyrosination promotes monolayer formation and apical trafficking in epithelial cells." in: Journal of cell science, Vol. 125, Issue Pt 24, pp. 5998-6008, (2013) (PubMed).
: "Mechanisms for axon maintenance and plasticity in motoneurons: alterations in motoneuron disease." in: Journal of anatomy, Vol. 224, Issue 1, pp. 3-14, (2013) (PubMed).
: "Structural basis of microtubule severing by the hereditary spastic paraplegia protein spastin." in: Nature, Vol. 451, Issue 7176, pp. 363-7, (2008) (PubMed).
: "Two catalytic domains are required for protein deacetylation." in: The Journal of biological chemistry, Vol. 281, Issue 5, pp. 2401-4, (2006) (PubMed).
: "Coordination of microtubule and microfilament dynamics by Drosophila Rho1, Spire and Cappuccino." in: Nature cell biology, Vol. 8, Issue 4, pp. 367-76, (2006) (PubMed).
: "Hypoxia stimulates carcinoma invasion by stabilizing microtubules and promoting the Rab11 trafficking of the alpha6beta4 integrin." in: Cancer research, Vol. 65, Issue 7, pp. 2761-9, (2005) (PubMed).
: "Monocilia on chicken embryonic endocardium in low shear stress areas." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 235, Issue 1, pp. 19-28, (2005) (PubMed).
: "Monoclonal antibody ID5: epitope characterization and minimal requirements for the recognition of polyglutamylated alpha- and beta-tubulin." in: European journal of cell biology, Vol. 78, Issue 1, pp. 15-20, (1999) (PubMed).
: "Primary cilia in cultured mammalian cells: detection with an antibody against detyrosinated alpha-tubulin (ID5) and by electron microscopy." in: Journal of submicroscopic cytology and pathology, Vol. 26, Issue 1, pp. 91-102, (1994) (PubMed).
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: "Tubulin detyrosination promotes monolayer formation and apical trafficking in epithelial cells." in: Journal of cell science, Vol. 125, Issue Pt 24, pp. 5998-6008, (2013) (PubMed).
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- Glu-alpha-Tubulin
抗原
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