(2 references)-
- 抗原
- GluN 2B
- 抗原表位
- AA 42-60
- 适用
- 小鼠, 大鼠
- 宿主
- 兔
- 克隆类型
- 多克隆
- 应用范围
- Immunoprecipitation (IP), Western Blotting (WB)
- 特异性
- Specific for GluN 2B
- 交叉反应 （详细）
- no cross-reactivity to GluN 2A.
- 纯化方法
- Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
- 免疫原
- Synthetic peptide (aa 42-60 of rat GluN 2B) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
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- 应用备注
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WB: 1 : 1000 (AP staining)
ICC: not tested yet
IHC: not tested yet - 说明
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IP: For most effective IP use the solubilization protocol described in the ELISA protocol. Consider that protein-protein interaction may be affected.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- 缓冲液
- PBS
- 注意事项
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
- 储存条件
- -20 °C
- 储存方法
- Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
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: "Parental THC exposure leads to compulsive heroin-seeking and altered striatal synaptic plasticity in the subsequent generation." in: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 39, Issue 6, pp. 1315-23, (2014) (PubMed).
: "Novel application of stem cell-derived neurons to evaluate the time- and dose-dependent progression of excitotoxic injury." in: PLoS ONE, Vol. 8, Issue 5, pp. e64423, (2013) (PubMed).
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: "Parental THC exposure leads to compulsive heroin-seeking and altered striatal synaptic plasticity in the subsequent generation." in: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 39, Issue 6, pp. 1315-23, (2014) (PubMed).
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- 抗原
- GluN 2B
- 背景
- Synonyms: NMDA-receptor 2B
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