PVALB 抗体 (Sulfo-Cyanine 3)
Quick Overview for PVALB 抗体 (Sulfo-Cyanine 3) (ABIN1742402)
抗原
See all PVALB 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse anti-Parvalbumin IgG
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特异性
- Specific for Parvalbumin.
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交叉反应 (详细)
- Reacts with: rat (P02625), mouse (P32848). No signal: zebrafish. Other species not tested yet.
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产品特性
- fluorescence-labeled with Cyanine3. Fluorescence labeled antibodies conjugated to (Sulfo-)Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy. Cyanine 2: λex 492 nm / λem 508 nm Sulfo-Cyanine 3: λex 554 nm / λem 568 nm Sulfo-Cyanine 5: λex 649 nm / λem 667 nm (Sulfo-)Cyanine 5 dyes are well suited for STORM high resolution microscopy. Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates.
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纯化方法
- purified IgG
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免疫原
- Full-length recombinant rat Parvalbumin (UniProt Id: P02625)
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亚型
- IgG1
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应用备注
- WB:N/A IP:N/A ICC:not tested yet IHC:1 : 200 up to 1 : 500, IHC-P:not tested yet
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说明
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WB: The polyclonal antibodies are highly recommended. Due to its small size, a tricine gel should be used. .
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.
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缓冲液
- PBS
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
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储存条件
- -20 °C,-80 °C
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储存方法
- Albumin and azide were added for stabilization. Reconstitute immediately upon receipt! Either add 1:1 (v/v) glycerol then aliquot and store at -20°C until use or store aliquots at -80°C without additives.
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- PVALB (Parvalbumin (PVALB))
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别名
- Parvalbumin
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背景
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Synonyms: Pvalb, Parv
Antigen Information: Parvalbumin is calcium binding protein specific for a subpopulation of GABAergic interneurones
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UniProt
- P02625
抗原
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