GFAP 抗体 (Biotin)
Quick Overview for GFAP 抗体 (Biotin) (ABIN1742362)
抗原
See all GFAP 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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原理
- Mouse anti-GFAP IgG
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交叉反应 (详细)
- Reacts with: human (P14136), rat (P47819), mouse (P03995), cow. Other species not tested yet.
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产品特性
- labeled with Biotin. Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems. The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known.
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纯化方法
- purified IgG
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免疫原
- full-length recombinant human GFAP (UniProt Id: P14136) Epitop AA 1 to 15 from human GFAP (UniProt Id: P14136)
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亚型
- IgG1
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应用备注
- WB:1 : 1000 , IP:yes ICC:not recommended IHC:not recommended IHC-P:not tested yet ELISA:yes
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.
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缓冲液
- PBS
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储存液
- Without preservative
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注意事项
- Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
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储存条件
- -20 °C,-80 °C,4 °C
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储存方法
- Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Do not freeze!
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: "A DNA-based nano-immunoassay for the label-free detection of glial fibrillary acidic protein in multicell lysates." in: Nanomedicine : nanotechnology, biology, and medicine, Vol. 11, Issue 2, pp. 293-300, (2015) (PubMed).
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: "A DNA-based nano-immunoassay for the label-free detection of glial fibrillary acidic protein in multicell lysates." in: Nanomedicine : nanotechnology, biology, and medicine, Vol. 11, Issue 2, pp. 293-300, (2015) (PubMed).
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- GFAP (Glial Fibrillary Acidic Protein (GFAP))
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别名
- GFAP
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背景
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Synonyms: Glial fibrillary acidic protein, Neuroinflamm-Astrocyt
Antigen Information: K.O. GFAP is an astrocyte-specific type-III intermediate filament protein
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UniProt
- P14136
抗原
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