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GFAP 抗体 (Biotin)

This anti-GFAP antibody is a 小鼠 单克隆 antibody detecting GFAP in WB, ELISA 和 IP. Suitable for 人, 大鼠, 小鼠 和 Cow. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN1742362
发货至: 中国

Quick Overview for GFAP 抗体 (Biotin) (ABIN1742362)

抗原

See all GFAP 抗体
GFAP (Glial Fibrillary Acidic Protein (GFAP))

适用

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人, 大鼠, 小鼠, Cow

宿主

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小鼠

克隆类型

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单克隆

标记

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This GFAP antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), ELISA, Immunoprecipitation (IP)

质量等级

KO Validated

克隆位点

186C6
  • 原理

    Mouse anti-GFAP IgG

    交叉反应 (详细)

    Reacts with: human (P14136), rat (P47819), mouse (P03995), cow. Other species not tested yet.

    产品特性

    labeled with Biotin. Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems. The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known.

    纯化方法

    purified IgG

    免疫原

    full-length recombinant human GFAP (UniProt Id: P14136) Epitop AA 1 to 15 from human GFAP (UniProt Id: P14136)

    亚型

    IgG1
  • 应用备注

    WB:1 : 1000 , IP:yes ICC:not recommended IHC:not recommended IHC-P:not tested yet ELISA:yes

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

    缓冲液

    PBS

    储存液

    Without preservative

    注意事项

    Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

    储存条件

    -20 °C,-80 °C,4 °C

    储存方法

    Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Do not freeze!
  • Ganau, Bosco, Palma, Corvaglia, Parisse, Fruk, Beltrami, Cesselli, Casalis, Scoles: "A DNA-based nano-immunoassay for the label-free detection of glial fibrillary acidic protein in multicell lysates." in: Nanomedicine : nanotechnology, biology, and medicine, Vol. 11, Issue 2, pp. 293-300, (2015) (PubMed).

  • 抗原

    GFAP (Glial Fibrillary Acidic Protein (GFAP))

    别名

    GFAP

    背景

    Synonyms: Glial fibrillary acidic protein, Neuroinflamm-Astrocyt

    Antigen Information: K.O. GFAP is an astrocyte-specific type-III intermediate filament protein

    UniProt

    P14136
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