Arc 抗体 (AA 1-214)
Quick Overview for Arc 抗体 (AA 1-214) (ABIN1742333)
抗原
See all Arc 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-214
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特异性
- Specific for Arc.
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纯化方法
- purified IgG. Azide was added before lyophilization.
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免疫原
- Strep-Tag® fusion protein of full-length mouse arc. Epitope Binds within first 214 aa.
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亚型
- IgG2b
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应用备注
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WB: not recommended
ICC: not tested yet
IHC: not tested yet -
说明
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ELISA: Suitable as capture antibody for sandwich-ELISA as detector antibody (protocol for sandwich-ELISA).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
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缓冲液
- PBS, 0.02% sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
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储存条件
- -20 °C
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储存方法
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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: "Application of hairless mouse strain to bioluminescence imaging of Arc expression in mouse brain." in: BMC neuroscience, Vol. 18, Issue 1, pp. 18, (2017) (PubMed).
: "Ethanol Toxicity During Brain Development: Alterations of Excitatory Synaptic Transmission in Immature Organotypic Hippocampal Slice Cultures." in: Alcoholism, clinical and experimental research, Vol. 40, Issue 4, pp. 706-16, (2016) (PubMed).
: "Enhanced expression of immediate-early genes in mouse hippocampus after trimethyltin treatment." in: Acta histochemica, (2016) (PubMed).
: "Vagus Nerve Stimulation Enhances Extinction of Conditioned Fear in Rats and Modulates Arc Protein, CaMKII, and GluN2B-Containing NMDA Receptors in the Basolateral Amygdala." in: Neural plasticity, Vol. 2016, pp. 4273280, (2016) (PubMed).
: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." in: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, (2012) (PubMed).
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: "Application of hairless mouse strain to bioluminescence imaging of Arc expression in mouse brain." in: BMC neuroscience, Vol. 18, Issue 1, pp. 18, (2017) (PubMed).
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- Arc (Activity-Regulated Cytoskeleton-Associated Protein (Arc))
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别名
- Arc
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背景
- Synonyms: Arg 3.1
抗原
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