Uncoating ATPase (AA 1-650) 抗体
This 小鼠 单克隆 antibody specifically detects Uncoating ATPase in ICC, IHC (p) 和 WB. It exhibits reactivity toward Cow, Hamster, 人, 小鼠 和 大鼠. It has been mentioned in 12+ publications
(12 references)
产品编号 ABIN1742323
发货至:
中国
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Quick Overview for Uncoating ATPase (AA 1-650) 抗体 (ABIN1742323)
抗原
Uncoating ATPase
适用
Cow, Hamster, 人, 小鼠, 大鼠
宿主
小鼠
克隆类型
单克隆
应用范围
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
克隆位点
3C5
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抗原表位
- AA 1-650
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原理
- Mouse anti-Uncoating ATPase IgG
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特异性
- Specific for uncoating ATPase.
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交叉反应 （详细）
- Reacts with: human (P11142), rat (P63018), mouse (P63017), hamster, cow. Other species not tested yet.
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纯化方法
- purified IgG
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免疫原
- Recombinant protein corresponding to AA 1 to 650 from bovine Uncoating ATPase (UniProt Id: P19120) Epitop AA 391 to 546 from bovine Uncoating ATPase (UniProt Id: P19120)
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亚型
- IgG1
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应用备注
- WB:1 : 500 up to 1 : 5000 IHC-P:1 : 200,
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说明
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For fixation a 20 min treatment with 3.7 % PFA in PBS is recommended. When microinjected into cells, the antibody inhibits clathrin mediated endocytosis.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.
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缓冲液
- PBS. Albumin and azide were added for stabilization.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze! Antibodies should be stored at +4°C when still lyophilized.
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储存条件
- -20 °C,-80 °C,4 °C
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储存方法
- Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!
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: "A mouse model for fucosidosis recapitulates storage pathology and neurological features of the milder form of the human disease." in: Disease models & mechanisms, Vol. 9, Issue 9, pp. 1015-28, (2016) (PubMed).
: "Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse." in: Frontiers in cellular neuroscience, Vol. 8, pp. 60, (2014) (PubMed).
: "Synaptic function of nicastrin in hippocampal neurons." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 24, pp. 8973-8, (2014) (PubMed).
: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).
: "The SNARE protein vti1a functions in dense-core vesicle biogenesis." in: The EMBO journal, Vol. 33, Issue 15, pp. 1681-97, (2014) (PubMed).
: "Cysteine string protein-alpha prevents activity-dependent degeneration in GABAergic synapses." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 21, pp. 7377-91, (2010) (PubMed).
: "Alpha-synuclein promotes SNARE-complex assembly in vivo and in vitro." in: Science (New York, N.Y.), Vol. 329, Issue 5999, pp. 1663-7, (2010) (PubMed).
: "Endosomal sorting of readily releasable synaptic vesicles." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 44, pp. 19055-60, (2010) (PubMed).
: "CSPα promotes SNARE-complex assembly by chaperoning SNAP-25 during synaptic activity." in: Nature cell biology, Vol. 13, Issue 1, pp. 30-9, (2010) (PubMed).
: "Dominant-interfering Hsc70 mutants disrupt multiple stages of the clathrin-coated vesicle cycle in vivo." in: The Journal of cell biology, Vol. 152, Issue 3, pp. 607-20, (2001) (PubMed).
: "ATP- and cytosol-dependent release of adaptor proteins from clathrin-coated vesicles: A dual role for Hsc70." in: Molecular biology of the cell, Vol. 9, Issue 8, pp. 2217-29, (1998) (PubMed).
: "Receptor-mediated endocytosis is sensitive to antibodies against the uncoating ATPase (hsc70)." in: Journal of cell science, Vol. 107 ( Pt 5), pp. 1185-96, (1994) (PubMed).
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- Uncoating ATPase
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背景
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Synonyms: hsc 70, Heat shock 70 kDa protein 8, hsc70
Antigen Information: Uncoating ATPase is involved in the removal of the clathrin coat of vesicles
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UniProt
- P19120
抗原
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