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Synaptojanin 1 抗体 (Isoform 2)

This anti-Synaptojanin 1 antibody is a 兔 多克隆 antibody detecting Synaptojanin 1 in WB 和 ICC. Suitable for 大鼠, 小鼠 和 Hamster. This Primary Antibody has been cited in 7+ publications.
产品编号 ABIN1742316
发货至: 中国

Quick Overview for Synaptojanin 1 抗体 (Isoform 2) (ABIN1742316)

抗原

See all Synaptojanin 1 (SYNJ1) 抗体
Synaptojanin 1 (SYNJ1)

适用

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大鼠, 小鼠, Hamster

宿主

  • 32
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克隆类型

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多克隆

标记

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This Synaptojanin 1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunocytochemistry (ICC)
  • 抗原表位

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    AA 1292-1308, Isoform 2

    特异性

    Recognizes all isoforms of synaptojanin 1.

    纯化方法

    Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

    免疫原

    Synthetic peptide CLPSDSSPQLQQEQP (aa 1292-1308 in isoform 2) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
  • 应用备注

    WB: 1 : 200 up to 1 : 2000 (AP staining)
    IP: not tested yet

    说明

    Running SDS-PAGE with non-boiled samples gives stronger signals.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

    缓冲液

    PBS

    注意事项

    Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

    储存条件

    -20 °C

    储存方法

    Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
  • Cliburn, Dunn, Stout, Hoffman, Lohr, Bernstein, Winokur, Burkett, Schmitz, Caudle, Miller: "Immunochemical localization of vesicular monoamine transporter 2 (VMAT2) in mouse brain." in: Journal of chemical neuroanatomy, (2016) (PubMed).

    Fuchs, Brandstätter, Regus-Leidig: "Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse." in: Frontiers in cellular neuroscience, Vol. 8, pp. 60, (2014) (PubMed).

    Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

    Park, Kim, Lee, Park, Park, Sun, Kim, Chang: "SNX18 shares a redundant role with SNX9 and modulates endocytic trafficking at the plasma membrane." in: Journal of cell science, Vol. 123, Issue Pt 10, pp. 1742-50, (2010) (PubMed).

    Uthaiah, Hudspeth: "Molecular anatomy of the hair cell's ribbon synapse." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 37, pp. 12387-99, (2010) (PubMed).

    Hoopmann, Punge, Barysch, Westphal, Bückers, Opazo, Bethani, Lauterbach, Hell, Rizzoli: "Endosomal sorting of readily releasable synaptic vesicles." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 44, pp. 19055-60, (2010) (PubMed).

    Shin, Lee, Ahn, Kim, Ahn, YongPark, Chang: "Sorting nexin 9 interacts with dynamin 1 and N-WASP and coordinates synaptic vesicle endocytosis." in: The Journal of biological chemistry, Vol. 282, Issue 39, pp. 28939-50, (2007) (PubMed).

  • 抗原

    Synaptojanin 1 (SYNJ1)

    别名

    Synaptojanin 1

    途径

    Inositol Metabolic Process, Synaptic Vesicle Exocytosis
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