Bassoon 抗体 (C-Term)
(7 references)Quick Overview for Bassoon 抗体 (C-Term) (ABIN1742310)
抗原
See all Bassoon (BSN) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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特异性
- Specific for bassoon.
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纯化方法
- hybridoma supernatant
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免疫原
- Recombinant protein containing the 330 C-terminal aa of rat bassoon.
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亚型
- IgG2b
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anti-Bassoon (BSN) antibody
BSN 适用: 大鼠, 小鼠 IHC, WB, ICC 宿主: 兔 Polyclonal unconjugated
anti-Bassoon (BSN) antibodyBSN 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Bassoon (BSN) (AA 786-1041) antibodyBSN 适用: 人 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Bassoon (BSN) (AA 786-1041) antibody (HRP)BSN 适用: 人 IHC, WB 宿主: 兔 Polyclonal HRP
anti-Bassoon (BSN) (AA 786-1041) antibody (FITC)BSN 适用: 人 IHC, WB 宿主: 兔 Polyclonal FITC
anti-Bassoon (BSN) (AA 786-1041) antibody (Biotin)BSN 适用: 人 IHC, WB 宿主: 兔 Polyclonal Biotin
anti-Bassoon (BSN) (AA 786-1041) antibody (PE)BSN 适用: 人 IHC, WB 宿主: 兔 Polyclonal PE
anti-Bassoon (BSN) (AA 786-1041) antibody (APC)BSN 适用: 人 IHC, WB 宿主: 兔 Polyclonal APC
anti-Bassoon (BSN) (AA 786-1041) antibody (PerCP)BSN 适用: 人 IHC, WB 宿主: 兔 Polyclonal PerCP
anti-Bassoon (BSN) (AA 786-1041) antibody (Atto 488)BSN 适用: 人 IHC, WB 宿主: 兔 Polyclonal Atto 488
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应用备注
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WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 100 up to 1 : 500
IHC: not tested yet -
说明
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ICC: Mild fixation conditions (2 % PFA for 20 min) are recommended. ELISA: Suitable as capture antibody for sandwich-ELISA as detector antibody (protocol for sandwich-ELISA).
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- PBS
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注意事项
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When hybridoma supernatant is reconstituted, we usually add small amounts of azide or thimerosal to prevent microbial growth. Ascites should be stored frozen (-20 °C). Monoclonals usually do not suffer from repetitive freeze-thawing but you may aliquot them into small samples to avoid too many freeze-thaw cycles.
Prolonged storage at 4 °C is not recommended! Unlike serum, ascites may contain proteases that will ultimately degrade the antibodies. Addition of protease inhibitors helps to slow degradation. -
储存条件
- -20 °C
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储存方法
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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: "Microglia contribute to circuit defects in Mecp2 null mice independent of microglia-specific loss of Mecp2 expression." in: eLife, Vol. 5, (2016) (PubMed).
: "Effects of trace metal profiles characteristic for autism on synapses in cultured neurons." in: Neural plasticity, Vol. 2015, pp. 985083, (2015) (PubMed).
: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).
: "Regulation of presynaptic anchoring of the scaffold protein Bassoon by phosphorylation-dependent interaction with 14-3-3 adaptor proteins." in: PLoS ONE, Vol. 8, Issue 3, pp. e58814, (2013) (PubMed).
: "Synapsin selectively controls the mobility of resting pool vesicles at hippocampal terminals." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 12, pp. 3969-80, (2012) (PubMed).
: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." in: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, (2012) (PubMed).
: "TRPM1 is required for the depolarizing light response in retinal ON-bipolar cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 45, pp. 19174-8, (2009) (PubMed).
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: "Microglia contribute to circuit defects in Mecp2 null mice independent of microglia-specific loss of Mecp2 expression." in: eLife, Vol. 5, (2016) (PubMed).
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- Bassoon (BSN)
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别名
- Bassoon
抗原
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