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Bassoon 抗体 (C-Term)

Cited in 7+ publications. This 小鼠 单克隆 anti-Bassoon antibody (Clone 219E1) specifically detects Bassoon in WB, ICC, IP 和 IHC (p). The antibody is reactive with 大鼠 和 小鼠 samples.
产品编号 ABIN1742310
发货至: 中国
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Quick Overview for Bassoon 抗体 (C-Term) (ABIN1742310)

抗原

See all Bassoon (BSN) 抗体
Bassoon (BSN)

适用

  • 23
  • 20
  • 13
大鼠, 小鼠

宿主

  • 24
  • 10
小鼠

克隆类型

  • 24
  • 10
单克隆

标记

  • 14
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Bassoon antibody is un-conjugated

应用范围

  • 27
  • 22
  • 10
  • 5
  • 3
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

219E1
  • 抗原表位

    • 13
    • 7
    • 2
    • 1
    • 1
    • 1
    C-Term

    原理

    Mouse anti-Bassoon supernatant

    特异性

    Specific for bassoon.

    交叉反应 (详细)

    Reacts with: rat (O88778), mouse (O88737). Other species not tested yet.

    纯化方法

    hybridoma supernatant

    免疫原

    Recombinant protein corresponding to residues near the carboxy terminus of rat Bassoon. (UniProt Id: O88778)

    亚型

    IgG2b
  • 应用备注

    WB:1 : 1000 , IP:yes ICC:1 : 100 up to 1 : 500, IHC:not recommended IHC-P:1 : 500, ELISA:

    说明

    ICC: Mild fixation conditions (2 % PFA for 20 min) are recommended. ELISA: Suitable as capture antibody for sandwich-ELISA as detector antibody (protocol for sandwich-ELISA).

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    For reconstitution add 200 μLH2O, then aliquot and store at -20 °C until use.

    注意事项

    Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

    储存条件

    -20 °C,4 °C

    储存方法

    Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
  • Schafer, Heller, Gunner, Heller, Gordon, Hammond, Wolf, Jung, Stevens: "Microglia contribute to circuit defects in Mecp2 null mice independent of microglia-specific loss of Mecp2 expression." in: eLife, Vol. 5, (2016) (PubMed).

    Hagmeyer, Mangus, Boeckers, Grabrucker: "Effects of trace metal profiles characteristic for autism on synapses in cultured neurons." in: Neural plasticity, Vol. 2015, pp. 985083, (2015) (PubMed).

    Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

    Schröder, Stellmacher, Romorini, Marini, Montenegro-Venegas, Altrock, Gundelfinger, Fejtova: "Regulation of presynaptic anchoring of the scaffold protein Bassoon by phosphorylation-dependent interaction with 14-3-3 adaptor proteins." in: PLoS ONE, Vol. 8, Issue 3, pp. e58814, (2013) (PubMed).

    Orenbuch, Shalev, Marra, Sinai, Lavy, Kahn, Burden, Staras, Gitler: "Synapsin selectively controls the mobility of resting pool vesicles at hippocampal terminals." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 12, pp. 3969-80, (2012) (PubMed).

    Vasileva, Horstmann, Geumann, Gitler, Kuner: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." in: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, (2012) (PubMed).

    Morgans, Zhang, Jeffrey, Nelson, Burke, Duvoisin, Brown: "TRPM1 is required for the depolarizing light response in retinal ON-bipolar cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 45, pp. 19174-8, (2009) (PubMed).

  • 抗原

    Bassoon (BSN)

    别名

    Bassoon

    背景

    Synonyms: Bsn

    Antigen Information: Bassoon is a large multi-domain protein of the presynaptic active zone

    UniProt

    O88778
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