Bassoon 抗体 (C-Term)
Quick Overview for Bassoon 抗体 (C-Term) (ABIN1742310)
抗原
See all Bassoon (BSN) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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原理
- Mouse anti-Bassoon supernatant
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特异性
- Specific for bassoon.
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交叉反应 (详细)
- Reacts with: rat (O88778), mouse (O88737). Other species not tested yet.
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纯化方法
- hybridoma supernatant
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免疫原
- Recombinant protein corresponding to residues near the carboxy terminus of rat Bassoon. (UniProt Id: O88778)
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亚型
- IgG2b
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应用备注
- WB:1 : 1000 , IP:yes ICC:1 : 100 up to 1 : 500, IHC:not recommended IHC-P:1 : 500, ELISA:
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说明
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ICC: Mild fixation conditions (2 % PFA for 20 min) are recommended. ELISA: Suitable as capture antibody for sandwich-ELISA as detector antibody (protocol for sandwich-ELISA).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 200 μLH2O, then aliquot and store at -20 °C until use.
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注意事项
- Do not freeze! Antibodies should be stored at +4°C when still lyophilized.
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储存条件
- -20 °C,4 °C
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储存方法
- Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
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: "Microglia contribute to circuit defects in Mecp2 null mice independent of microglia-specific loss of Mecp2 expression." in: eLife, Vol. 5, (2016) (PubMed).
: "Effects of trace metal profiles characteristic for autism on synapses in cultured neurons." in: Neural plasticity, Vol. 2015, pp. 985083, (2015) (PubMed).
: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).
: "Regulation of presynaptic anchoring of the scaffold protein Bassoon by phosphorylation-dependent interaction with 14-3-3 adaptor proteins." in: PLoS ONE, Vol. 8, Issue 3, pp. e58814, (2013) (PubMed).
: "Synapsin selectively controls the mobility of resting pool vesicles at hippocampal terminals." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 12, pp. 3969-80, (2012) (PubMed).
: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." in: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, (2012) (PubMed).
: "TRPM1 is required for the depolarizing light response in retinal ON-bipolar cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 45, pp. 19174-8, (2009) (PubMed).
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: "Microglia contribute to circuit defects in Mecp2 null mice independent of microglia-specific loss of Mecp2 expression." in: eLife, Vol. 5, (2016) (PubMed).
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- Bassoon (BSN)
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别名
- Bassoon
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背景
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Synonyms: Bsn
Antigen Information: Bassoon is a large multi-domain protein of the presynaptic active zone
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UniProt
- O88778
抗原
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