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NSF 抗体 (AA 733-744)

This anti-NSF antibody is a 兔 多克隆 antibody detecting NSF in WB, ELISA, IP, IHC 和 ICC. Suitable for 人, 大鼠, 小鼠 和 Hamster. This Primary Antibody has been cited in 5+ publications.
产品编号 ABIN1742270
发货至: 中国

Quick Overview for NSF 抗体 (AA 733-744) (ABIN1742270)

抗原

See all NSF 抗体
NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

适用

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人, 大鼠, 小鼠, Hamster

宿主

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克隆类型

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多克隆

标记

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This NSF antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
  • 抗原表位

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    AA 733-744

    特异性

    Specific for NSF.

    纯化方法

    antiserum

    免疫原

    Synthetic peptide REEGASPLDFD (aa 733-744 in rat NSF) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
  • 应用备注

    WB: 1 : 1000 (AP staining)
    ICC: 1 : 100 up to 1 : 1000
    IHC: 1 : 200

    说明

    ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742269 as capture antibody (protocol for sandwich-ELISA).

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    PBS

    注意事项

    Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
    Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

    储存条件

    4 °C/-20 °C

    储存方法

    Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
  • de Paola, Bello, Michaut: "Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes." in: PLoS ONE, Vol. 10, Issue 8, pp. e0135679, (2015) (PubMed).

    Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

    Tian, Wu, Unzicker, Lu, Cai, Li, Schirra, Matti, Stevens, Deng, Rettig, Sheng: "The role of Snapin in neurosecretion: snapin knock-out mice exhibit impaired calcium-dependent exocytosis of large dense-core vesicles in chromaffin cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 25, Issue 45, pp. 10546-55, (2005) (PubMed).

    Tailleux, Neyrolles, Honoré-Bouakline, Perret, Sanchez, Abastado, Lagrange, Gluckman, Rosenzwajg, Herrmann: "Constrained intracellular survival of Mycobacterium tuberculosis in human dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 4, pp. 1939-48, (2003) (PubMed).

    Michaut, Tomes, De Blas, Yunes, Mayorga: "Calcium-triggered acrosomal exocytosis in human spermatozoa requires the coordinated activation of Rab3A and N-ethylmaleimide-sensitive factor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 18, pp. 9996-10001, (2000) (PubMed).

  • 抗原

    NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

    别名

    NSF
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