电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

Complexin 1, 2 (AA 45-81) 抗体

适用: 小鼠, 大鼠, 人, Cow, 斑马鱼 ICC, WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN1742265
发货至: 中国
  • 抗原
    Complexin 1, 2
    抗原表位
    • 1
    • 1
    • 1
    AA 45-81
    适用
    小鼠, 大鼠, 人, Cow, 斑马鱼
    宿主
    • 1
    • 1
    • 1
    克隆类型
    • 2
    • 1
    多克隆
    标记
    • 3
    非结合性
    应用范围
    Immunocytochemistry (ICC), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
    特异性
    Recognizes complexin 1 and 2.
    纯化方法
    antiserum
    免疫原
    Synthetic peptide EEERKAKHARMEAEREKVRQQIRDKYGLKKKEEKEAE (aa 45-81 in complexin 2) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue. Immunogen is located inside the mapped binding domain of complexin 2 to the SNARE complex (aa 41 - 91).
  • 应用备注
    WB: 1 : 1000 up to 1 : 20000 (AP staining)
    ICC: 1 : 200 up to 1 : 500
    说明

    IP: Co-immunoprecipitates the SNARE complex.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    PBS
    注意事项
    Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
    Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.
    储存条件
    4 °C/-20 °C
    储存方法
    Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
  • Getz, Visser, Bell, Xu, Flynn, Zaidi, Syed: "Two proteolytic fragments of menin coordinate the nuclear transcription and postsynaptic clustering of neurotransmitter receptors during synaptogenesis between Lymnaea neurons." in: Scientific reports, Vol. 6, pp. 31779, (2016) (PubMed).

    Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

    Tsai, Brewis, van Maaren, Gadella: "Involvement of complexin 2 in docking, locking and unlocking of different SNARE complexes during sperm capacitation and induced acrosomal exocytosis." in: PLoS ONE, Vol. 7, Issue 3, pp. e32603, (2012) (PubMed).

    Lee, Brecha: "Immunocytochemical evidence for SNARE protein-dependent transmitter release from guinea pig horizontal cells." in: The European journal of neuroscience, Vol. 31, Issue 8, pp. 1388-401, (2010) (PubMed).

    Falkowski, Thomas, Groblewski: "Complexin 2 modulates vesicle-associated membrane protein (VAMP) 2-regulated zymogen granule exocytosis in pancreatic acini." in: The Journal of biological chemistry, Vol. 285, Issue 46, pp. 35558-66, (2010) (PubMed).

  • 抗原
    Complexin 1, 2
    背景
    Synonyms: Synaphin 2,1, CPX 1, CPX 2
You are here:
客服