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SV2B 抗体 (AA 2-17)

This anti-SV2B antibody is a 小鼠 单克隆 antibody detecting SV2B in WB, IHC, ICC 和 IP. Suitable for 人, 小鼠 和 大鼠. This Primary Antibody has been cited in 2+ publications.
产品编号 ABIN1742257
发货至: 中国

Quick Overview for SV2B 抗体 (AA 2-17) (ABIN1742257)

抗原

See all SV2B 抗体
SV2B (Synaptic Vesicle Glycoprotein 2B (SV2B))

适用

  • 36
  • 23
  • 7
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
人, 小鼠, 大鼠

宿主

  • 31
  • 4
  • 1
  • 1
小鼠

克隆类型

  • 36
  • 1
单克隆

标记

  • 17
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This SV2B antibody is un-conjugated

应用范围

  • 30
  • 15
  • 13
  • 13
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

克隆位点

246E8
  • 抗原表位

    • 15
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    AA 2-17

    特异性

    Specific for SV2 B.

    纯化方法

    purified IgG

    免疫原

    Synthetic peptide DDYRYRDNYEGYAPND (aa 2-17 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

    亚型

    IgG2b
  • 应用备注

    WB: 1 : 1000 (AP staining)
    ICC: 1 : 100 up to 1 : 500
    IHC: 1 : 200
    IHC-P: 1 : 500

    说明

    WB: SV2 aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

    缓冲液

    PBS, 0.02% sodium azide (= 0.02 µg)

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

    储存条件

    -20 °C

    储存方法

    Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
  • Aroeira, Vaz, Sebastião, Valente: "BDNF modulates glycine uptake in hippocampal synaptosomes by decreasing membrane insertion of glycine transporter 2." in: Neurochemistry international, Vol. 99, pp. 94-102, (2016) (PubMed).

    Dagar, Nagar, Goel, Cherukuri, Dhingra: "Loss of photoreceptors results in upregulation of synaptic proteins in bipolar cells and amacrine cells." in: PLoS ONE, Vol. 9, Issue 3, pp. e90250, (2014) (PubMed).

  • 抗原

    SV2B (Synaptic Vesicle Glycoprotein 2B (SV2B))

    别名

    SV2 B
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